Genomic diversity of Bartonella henselae isolates from domestic cats from Japan, the USA and France by pulsed-field gel electrophoresis

Soichi Maruyama, Rickie W. Kasten, Henri Jean Boulouis, Nikos A. Gurfield, Yasuji Katsube, Bruno B Chomel

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The genomic DNA diversity of 27 Bartonella henselae and three B. clarridgeiae isolates from 18 domestic cats from Japan, the USA and France was investigated by pulsed-field gel electrophoresis (PFGE) with NotI, AscI and SmaI restriction enzymes. A great diversity of genomic patterns was found for all B. henselae, but none for B. clarridgeiae isolates. The DNA size of B. henselae and B. clarridgeiae isolates were 1.7-2.9 and 1.7 Mbp, respectively. All 13 Japanese cat isolates were identified as B. henselae type I. Furthermore, three of the four Japanese cats harbored genetically different B. henselae type I isolates, suggesting for the first time co-infection with various type I isolates. One French cat and one American cat were co-infected with B. henselae and B. clarridgeiae. B. henselae type I and type II were mainly grouped in two different clusters by PFGE using SmaI endonuclease in the dendrogram.

Original languageEnglish (US)
Pages (from-to)337-349
Number of pages13
JournalVeterinary Microbiology
Volume79
Issue number4
DOIs
StatePublished - Apr 19 2001

Fingerprint

Bartonella henselae
Pulsed Field Gel Electrophoresis
pulsed-field gel electrophoresis
France
Japan
Cats
cats
genomics
Bartonella clarridgeiae
asci
Endonucleases
DNA
Coinfection
mixed infection
Enzymes
enzymes

Keywords

  • Bartonella clarridgeiae
  • Bartonella henselae
  • Cat
  • Genomic diversity
  • Pulsed-field gel electrophoresis

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

Cite this

Genomic diversity of Bartonella henselae isolates from domestic cats from Japan, the USA and France by pulsed-field gel electrophoresis. / Maruyama, Soichi; Kasten, Rickie W.; Boulouis, Henri Jean; Gurfield, Nikos A.; Katsube, Yasuji; Chomel, Bruno B.

In: Veterinary Microbiology, Vol. 79, No. 4, 19.04.2001, p. 337-349.

Research output: Contribution to journalArticle

Maruyama, Soichi ; Kasten, Rickie W. ; Boulouis, Henri Jean ; Gurfield, Nikos A. ; Katsube, Yasuji ; Chomel, Bruno B. / Genomic diversity of Bartonella henselae isolates from domestic cats from Japan, the USA and France by pulsed-field gel electrophoresis. In: Veterinary Microbiology. 2001 ; Vol. 79, No. 4. pp. 337-349.
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