Abstract
The genomic DNA diversity of 27 Bartonella henselae and three B. clarridgeiae isolates from 18 domestic cats from Japan, the USA and France was investigated by pulsed-field gel electrophoresis (PFGE) with NotI, AscI and SmaI restriction enzymes. A great diversity of genomic patterns was found for all B. henselae, but none for B. clarridgeiae isolates. The DNA size of B. henselae and B. clarridgeiae isolates were 1.7-2.9 and 1.7 Mbp, respectively. All 13 Japanese cat isolates were identified as B. henselae type I. Furthermore, three of the four Japanese cats harbored genetically different B. henselae type I isolates, suggesting for the first time co-infection with various type I isolates. One French cat and one American cat were co-infected with B. henselae and B. clarridgeiae. B. henselae type I and type II were mainly grouped in two different clusters by PFGE using SmaI endonuclease in the dendrogram.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 337-349 |
| Number of pages | 13 |
| Journal | Veterinary Microbiology |
| Volume | 79 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 19 2001 |
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Keywords
- Bartonella clarridgeiae
- Bartonella henselae
- Cat
- Genomic diversity
- Pulsed-field gel electrophoresis
ASJC Scopus subject areas
- Animal Science and Zoology
- Microbiology
- veterinary(all)
Cite this
Genomic diversity of Bartonella henselae isolates from domestic cats from Japan, the USA and France by pulsed-field gel electrophoresis. / Maruyama, Soichi; Kasten, Rickie W.; Boulouis, Henri Jean; Gurfield, Nikos A.; Katsube, Yasuji; Chomel, Bruno B.
In: Veterinary Microbiology, Vol. 79, No. 4, 19.04.2001, p. 337-349.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genomic diversity of Bartonella henselae isolates from domestic cats from Japan, the USA and France by pulsed-field gel electrophoresis
AU - Maruyama, Soichi
AU - Kasten, Rickie W.
AU - Boulouis, Henri Jean
AU - Gurfield, Nikos A.
AU - Katsube, Yasuji
AU - Chomel, Bruno B
PY - 2001/4/19
Y1 - 2001/4/19
N2 - The genomic DNA diversity of 27 Bartonella henselae and three B. clarridgeiae isolates from 18 domestic cats from Japan, the USA and France was investigated by pulsed-field gel electrophoresis (PFGE) with NotI, AscI and SmaI restriction enzymes. A great diversity of genomic patterns was found for all B. henselae, but none for B. clarridgeiae isolates. The DNA size of B. henselae and B. clarridgeiae isolates were 1.7-2.9 and 1.7 Mbp, respectively. All 13 Japanese cat isolates were identified as B. henselae type I. Furthermore, three of the four Japanese cats harbored genetically different B. henselae type I isolates, suggesting for the first time co-infection with various type I isolates. One French cat and one American cat were co-infected with B. henselae and B. clarridgeiae. B. henselae type I and type II were mainly grouped in two different clusters by PFGE using SmaI endonuclease in the dendrogram.
AB - The genomic DNA diversity of 27 Bartonella henselae and three B. clarridgeiae isolates from 18 domestic cats from Japan, the USA and France was investigated by pulsed-field gel electrophoresis (PFGE) with NotI, AscI and SmaI restriction enzymes. A great diversity of genomic patterns was found for all B. henselae, but none for B. clarridgeiae isolates. The DNA size of B. henselae and B. clarridgeiae isolates were 1.7-2.9 and 1.7 Mbp, respectively. All 13 Japanese cat isolates were identified as B. henselae type I. Furthermore, three of the four Japanese cats harbored genetically different B. henselae type I isolates, suggesting for the first time co-infection with various type I isolates. One French cat and one American cat were co-infected with B. henselae and B. clarridgeiae. B. henselae type I and type II were mainly grouped in two different clusters by PFGE using SmaI endonuclease in the dendrogram.
KW - Bartonella clarridgeiae
KW - Bartonella henselae
KW - Cat
KW - Genomic diversity
KW - Pulsed-field gel electrophoresis
UR - http://www.scopus.com/inward/record.url?scp=0035912287&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035912287&partnerID=8YFLogxK
U2 - 10.1016/S0378-1135(00)00365-5
DO - 10.1016/S0378-1135(00)00365-5
M3 - Article
VL - 79
SP - 337
EP - 349
JO - Veterinary Microbiology
JF - Veterinary Microbiology
SN - 0378-1135
IS - 4
ER -