TY - JOUR
T1 - Genomic diversity of Bartonella henselae isolates from domestic cats from Japan, the USA and France by pulsed-field gel electrophoresis
AU - Maruyama, Soichi
AU - Kasten, Rickie W.
AU - Boulouis, Henri Jean
AU - Gurfield, Nikos A.
AU - Katsube, Yasuji
AU - Chomel, Bruno B
PY - 2001/4/19
Y1 - 2001/4/19
N2 - The genomic DNA diversity of 27 Bartonella henselae and three B. clarridgeiae isolates from 18 domestic cats from Japan, the USA and France was investigated by pulsed-field gel electrophoresis (PFGE) with NotI, AscI and SmaI restriction enzymes. A great diversity of genomic patterns was found for all B. henselae, but none for B. clarridgeiae isolates. The DNA size of B. henselae and B. clarridgeiae isolates were 1.7-2.9 and 1.7 Mbp, respectively. All 13 Japanese cat isolates were identified as B. henselae type I. Furthermore, three of the four Japanese cats harbored genetically different B. henselae type I isolates, suggesting for the first time co-infection with various type I isolates. One French cat and one American cat were co-infected with B. henselae and B. clarridgeiae. B. henselae type I and type II were mainly grouped in two different clusters by PFGE using SmaI endonuclease in the dendrogram.
AB - The genomic DNA diversity of 27 Bartonella henselae and three B. clarridgeiae isolates from 18 domestic cats from Japan, the USA and France was investigated by pulsed-field gel electrophoresis (PFGE) with NotI, AscI and SmaI restriction enzymes. A great diversity of genomic patterns was found for all B. henselae, but none for B. clarridgeiae isolates. The DNA size of B. henselae and B. clarridgeiae isolates were 1.7-2.9 and 1.7 Mbp, respectively. All 13 Japanese cat isolates were identified as B. henselae type I. Furthermore, three of the four Japanese cats harbored genetically different B. henselae type I isolates, suggesting for the first time co-infection with various type I isolates. One French cat and one American cat were co-infected with B. henselae and B. clarridgeiae. B. henselae type I and type II were mainly grouped in two different clusters by PFGE using SmaI endonuclease in the dendrogram.
KW - Bartonella clarridgeiae
KW - Bartonella henselae
KW - Cat
KW - Genomic diversity
KW - Pulsed-field gel electrophoresis
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U2 - 10.1016/S0378-1135(00)00365-5
DO - 10.1016/S0378-1135(00)00365-5
M3 - Article
C2 - 11267793
AN - SCOPUS:0035912287
VL - 79
SP - 337
EP - 349
JO - Veterinary Microbiology
JF - Veterinary Microbiology
SN - 0378-1135
IS - 4
ER -