Genomic cloning and characterization of the human eukaryotic initiation factor-2β promoter

John A. Chiorini, Suzanne Miyamoto, Stephanie J. Harkin, Brian Safer

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The translation initiation factor eIF2 consists of three subunits that are present in equal molar amounts. The genomic DNA containing the gene for eIF2β and its promoter were cloned and sequenced to characterize further the mechanism of their regulated synthesis. Whereas Southern blot analysis indicated that a number of copies of the gene may exist, only one full- length intron-containing copy was identified. Similar to the eIF2α promoter, the eIF2β promoter is TATA-less, CAAT-less, and GC-rich and contains an α- Pal binding motif. Mutation of the α-Pal binding sequence resulted in an 8- fold decrease in activity when assayed by the luciferase reporter gene constructs. The data suggest a common mechanism of transcriptional control for the two cloned subunits of eIF2.

Original languageEnglish (US)
Pages (from-to)4195-4201
Number of pages7
JournalJournal of Biological Chemistry
Volume274
Issue number7
DOIs
StatePublished - Feb 12 1999
Externally publishedYes

Fingerprint

Eukaryotic Initiation Factor-2
Peptide Initiation Factors
Gene Dosage
Cloning
Southern Blotting
Luciferases
Reporter Genes
Introns
Organism Cloning
Genes
Mutation
DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Genomic cloning and characterization of the human eukaryotic initiation factor-2β promoter. / Chiorini, John A.; Miyamoto, Suzanne; Harkin, Stephanie J.; Safer, Brian.

In: Journal of Biological Chemistry, Vol. 274, No. 7, 12.02.1999, p. 4195-4201.

Research output: Contribution to journalArticle

Chiorini, John A. ; Miyamoto, Suzanne ; Harkin, Stephanie J. ; Safer, Brian. / Genomic cloning and characterization of the human eukaryotic initiation factor-2β promoter. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 7. pp. 4195-4201.
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