Genomic and antigenic characterization of a cytopathic bovine viral diarrhea virus 1i isolated in the United States

John D. Neill, Beate Crossley, A. C. Mosena, Julia F. Ridpath, Darrell O. Bayles, Sharon K. Hietala, Mary Lea Killian, Shollie M. Falkenberg

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1 Scopus citations

Abstract

Bovine viral diarrhea viruses (BVDV) are a common global viral pathogen of ruminants. Considerable genetic variability is found amongst BVDV1 isolates, with at least 21 subgenotypes being described. In the United States, BVDV1a and 1b are the only subgenotypes described to date. Here, the genomic sequence of CA2005, a cytopathic BVDV1, was determined. This virus, isolated in California, did not segregate into either BVDV1a or 1b subgenotypes. BLAST analysis showed CA2005 was most closely related to BVDV1i isolates. CA2005 was also the first cytopathic BVDV1i and one of few non-1a, non-1b cytopathic viruses reported. The genomic sequence was 15,752 nucleotides in length. Cytopathogenicity was conferred by duplication of the NS3 protein with a small ubiquitin B insertion at the border of the NS2/NS3 proteins. Virus neutralization assays using antisera against BVDV1a vaccine viruses revealed variable neutralization, suggesting modified live vaccines may not be totally protective against CA2005 and similar viruses.

Original languageEnglish (US)
Pages (from-to)279-282
Number of pages4
JournalVirology
Volume535
DOIs
StatePublished - Sep 1 2019

Keywords

  • Antigenic cross-reactivity
  • Cytopathology
  • Emerging virus
  • Genomic duplication

ASJC Scopus subject areas

  • Virology

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    Neill, J. D., Crossley, B., Mosena, A. C., Ridpath, J. F., Bayles, D. O., Hietala, S. K., Killian, M. L., & Falkenberg, S. M. (2019). Genomic and antigenic characterization of a cytopathic bovine viral diarrhea virus 1i isolated in the United States. Virology, 535, 279-282. https://doi.org/10.1016/j.virol.2019.07.020