Abstract
To investigate the viral features of long-term nonprogressive HIV-1 infection and the selection of viral genomes, we studied serial complete HIV-1 sequences obtained from a mother-child pair, both long-term nonprogressors. Analysis of four genomic sequences demonstrated that all viral genes were intact, lacking major deletions or premature stop codons to easily explain the slow disease progression. These data suggest that viral attenuation, if present, was caused by subtle sequence variations or virus-host interactions. Serial sequences from an HIV-1-infected mother-child pair afforded us the opportunity to examine the immune selection of HIV-1 sequences years after transmission between individuals. We demonstrated that the daughter's strains were most likely subjected to immunoselection or immunoediting according to the presence of novel MHC class I alleles that differed between mother and daughter. An analysis of nef-specific cytotoxic T-lymphocyte responses in the child, whose HIV-1 nef sequence differed from the maternal nef, supported this interpretation. This study highlights the potential of full genome analysis in the investigation of pathogenesis and immune selection during HIV-1 evolution.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 309-315 |
| Number of pages | 7 |
| Journal | AIDS Research and Human Retroviruses |
| Volume | 23 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2007 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Immunology
- Virology
Cite this
Genomic analysis of HIV type 1 strains derived from a mother and child pair of long-term nonprogressors. / Reinis, Milan; Weiser, Barbara; Kuiken, Carla; Dong, Tao; Lang, Dorothy; Nachman, Sharon; Zhang, Yonghong; Rowland-Jones, Sarah; Burger, Harold.
In: AIDS Research and Human Retroviruses, Vol. 23, No. 2, 02.2007, p. 309-315.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genomic analysis of HIV type 1 strains derived from a mother and child pair of long-term nonprogressors
AU - Reinis, Milan
AU - Weiser, Barbara
AU - Kuiken, Carla
AU - Dong, Tao
AU - Lang, Dorothy
AU - Nachman, Sharon
AU - Zhang, Yonghong
AU - Rowland-Jones, Sarah
AU - Burger, Harold
PY - 2007/2
Y1 - 2007/2
N2 - To investigate the viral features of long-term nonprogressive HIV-1 infection and the selection of viral genomes, we studied serial complete HIV-1 sequences obtained from a mother-child pair, both long-term nonprogressors. Analysis of four genomic sequences demonstrated that all viral genes were intact, lacking major deletions or premature stop codons to easily explain the slow disease progression. These data suggest that viral attenuation, if present, was caused by subtle sequence variations or virus-host interactions. Serial sequences from an HIV-1-infected mother-child pair afforded us the opportunity to examine the immune selection of HIV-1 sequences years after transmission between individuals. We demonstrated that the daughter's strains were most likely subjected to immunoselection or immunoediting according to the presence of novel MHC class I alleles that differed between mother and daughter. An analysis of nef-specific cytotoxic T-lymphocyte responses in the child, whose HIV-1 nef sequence differed from the maternal nef, supported this interpretation. This study highlights the potential of full genome analysis in the investigation of pathogenesis and immune selection during HIV-1 evolution.
AB - To investigate the viral features of long-term nonprogressive HIV-1 infection and the selection of viral genomes, we studied serial complete HIV-1 sequences obtained from a mother-child pair, both long-term nonprogressors. Analysis of four genomic sequences demonstrated that all viral genes were intact, lacking major deletions or premature stop codons to easily explain the slow disease progression. These data suggest that viral attenuation, if present, was caused by subtle sequence variations or virus-host interactions. Serial sequences from an HIV-1-infected mother-child pair afforded us the opportunity to examine the immune selection of HIV-1 sequences years after transmission between individuals. We demonstrated that the daughter's strains were most likely subjected to immunoselection or immunoediting according to the presence of novel MHC class I alleles that differed between mother and daughter. An analysis of nef-specific cytotoxic T-lymphocyte responses in the child, whose HIV-1 nef sequence differed from the maternal nef, supported this interpretation. This study highlights the potential of full genome analysis in the investigation of pathogenesis and immune selection during HIV-1 evolution.
UR - http://www.scopus.com/inward/record.url?scp=33947202309&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33947202309&partnerID=8YFLogxK
U2 - 10.1089/aid.2006.0180
DO - 10.1089/aid.2006.0180
M3 - Article
C2 - 17331038
AN - SCOPUS:33947202309
VL - 23
SP - 309
EP - 315
JO - AIDS Research and Human Retroviruses
JF - AIDS Research and Human Retroviruses
SN - 0889-2229
IS - 2
ER -