Genome-Wide Search and Identification of a Novel Gel-Forming Mucin MUC19/Muc19 in Glandular Tissues

Yin Chen, Yu Hua Zhao, Tejas Baba Kalaslavadi, Edward Hamati, Keith Nehrke, Anh Dao Le, David K. Ann, Reen Wu

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

Gel-forming mucins are major contributors to the viscoelastic properties of mucus secretion. Currently, four gel-forming mucin genes have been identified: MUC2, MUC5AC, MUCSB, and MUC6. All these genes have five major cysteine-rich domains (four von Willebrand factor [vWF] C or D domains and one Cystine-knot [CT] domain) as their distinctive features, in contrast to other non-gel-forming type of mucins. The CT domain is believed to be involved in the initial mucin dimer formation and have very succinct relationship between different gel-forming mucins across different species. Because of gene duplication and evolutional modification, it is very likely that other gel-forming mucin genes exist. To search for new gel-forming mucin candidate genes, a "Hidden Markov Model"(HMM) was built from the common features of the CT domains of those gel-forming mucins. By using this model to screen all protein databases as well as the six-frame translated expression sequence tag and translated human genomic databases, we identified a locus located at the peri-centromere region of human chromosome 12 and the corresponding homologous region of mouse chromosome 15. We cloned the 3′ end of this gene and its mouse homolog. We found one vWF C domain, one CT domain, and various mucin-like threonine/serine-rich repeats. Phylogenetic analysis indicated the close relationship between this gene and the submaxillary mucin from porcine and bovine. A polydispersed signal was observed on the Northern blot, which indicates very large mRNA size. Further analysis of the upstream genomic sequences generated from human and mouse genome projects revealed three additional vWF D domains and many mucin-like threonine/serine- rich repeats. The expression of this gene is restricted to the mucous cells of various glandular tissues, including sublingual gland, submandibular gland, and submucosal gland of the trachea. Based on the chronological convention, we have given the name MUC19 to the human ortholog and Muc19 to the mouse.

Original languageEnglish (US)
Pages (from-to)155-165
Number of pages11
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume30
Issue number2
DOIs
StatePublished - Feb 2004

Fingerprint

Mucins
Genes
Gels
Genome
Tissue
Cystine
von Willebrand Factor
Threonine
Chromosomes
Serine
Sublingual Gland
Human Genome Project
Chromosomes, Human, Pair 15
Chromosomes, Human, Pair 12
Protein Databases
Gene Duplication
Centromere
Submandibular Gland
Human Chromosomes
Mucus

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology

Cite this

Genome-Wide Search and Identification of a Novel Gel-Forming Mucin MUC19/Muc19 in Glandular Tissues. / Chen, Yin; Zhao, Yu Hua; Kalaslavadi, Tejas Baba; Hamati, Edward; Nehrke, Keith; Le, Anh Dao; Ann, David K.; Wu, Reen.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 30, No. 2, 02.2004, p. 155-165.

Research output: Contribution to journalArticle

Chen, Yin ; Zhao, Yu Hua ; Kalaslavadi, Tejas Baba ; Hamati, Edward ; Nehrke, Keith ; Le, Anh Dao ; Ann, David K. ; Wu, Reen. / Genome-Wide Search and Identification of a Novel Gel-Forming Mucin MUC19/Muc19 in Glandular Tissues. In: American Journal of Respiratory Cell and Molecular Biology. 2004 ; Vol. 30, No. 2. pp. 155-165.
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