Genome-wide screening of alpha-tocopherol sensitive genes in heart tissue from alpha-tocopherol transfer protein null mice (ATTP-/-)

Vihas T. Vasu, Brad Hobson, Kishorchandra Gohil, Carroll E Cross

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Alpha-tocopherol transfer protein (ATTP) null mice (ATTP-/-) have a systemic deficiency of alpha-tocopherol (AT). The heart AT levels of ATTP-/- are <10% of those in ATTP+/+ mice. The genomic responses of heart to AT deficiency were determined in 3 months old male ATTP-/- mice and compared with their ATTP+/+ littermate controls using Affymetrix 430A 2.0 high density oligonucleotide arrays. Differential analysis of ∼13 000 genes identified repression of genes related to immune system and activation of genes related to lipid metabolism and inflammation with no significant change in the expression of classical antioxidant genes (catalase, superoxide dismutase, glutathione peroxidase) in ATTP-/- as compared to ATTP+/+ mice. The present data identifies novel classes of AT sensitive genes in heart tissue.

Original languageEnglish (US)
Pages (from-to)1572-1578
Number of pages7
JournalFEBS Letters
Volume581
Issue number8
DOIs
StatePublished - Apr 17 2007

Fingerprint

alpha-Tocopherol
Screening
Genes
Genome
Tissue
alpha-tocopherol transfer protein
Immune system
Glutathione Peroxidase
Oligonucleotide Array Sequence Analysis
Lipid Metabolism
Oligonucleotides
Catalase
Transcriptional Activation
Superoxide Dismutase
Immune System
Antioxidants
Chemical activation
Inflammation

Keywords

  • Alpha-tocopherol
  • Alpha-tocopherol transfer protein null mice
  • Gene expression profiling
  • Heart

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Genome-wide screening of alpha-tocopherol sensitive genes in heart tissue from alpha-tocopherol transfer protein null mice (ATTP-/-). / Vasu, Vihas T.; Hobson, Brad; Gohil, Kishorchandra; Cross, Carroll E.

In: FEBS Letters, Vol. 581, No. 8, 17.04.2007, p. 1572-1578.

Research output: Contribution to journalArticle

@article{05e9ec1dbcbc4389b7ca914979bef1a5,
title = "Genome-wide screening of alpha-tocopherol sensitive genes in heart tissue from alpha-tocopherol transfer protein null mice (ATTP-/-)",
abstract = "Alpha-tocopherol transfer protein (ATTP) null mice (ATTP-/-) have a systemic deficiency of alpha-tocopherol (AT). The heart AT levels of ATTP-/- are <10{\%} of those in ATTP+/+ mice. The genomic responses of heart to AT deficiency were determined in 3 months old male ATTP-/- mice and compared with their ATTP+/+ littermate controls using Affymetrix 430A 2.0 high density oligonucleotide arrays. Differential analysis of ∼13 000 genes identified repression of genes related to immune system and activation of genes related to lipid metabolism and inflammation with no significant change in the expression of classical antioxidant genes (catalase, superoxide dismutase, glutathione peroxidase) in ATTP-/- as compared to ATTP+/+ mice. The present data identifies novel classes of AT sensitive genes in heart tissue.",
keywords = "Alpha-tocopherol, Alpha-tocopherol transfer protein null mice, Gene expression profiling, Heart",
author = "Vasu, {Vihas T.} and Brad Hobson and Kishorchandra Gohil and Cross, {Carroll E}",
year = "2007",
month = "4",
day = "17",
doi = "10.1016/j.febslet.2007.03.017",
language = "English (US)",
volume = "581",
pages = "1572--1578",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "8",

}

TY - JOUR

T1 - Genome-wide screening of alpha-tocopherol sensitive genes in heart tissue from alpha-tocopherol transfer protein null mice (ATTP-/-)

AU - Vasu, Vihas T.

AU - Hobson, Brad

AU - Gohil, Kishorchandra

AU - Cross, Carroll E

PY - 2007/4/17

Y1 - 2007/4/17

N2 - Alpha-tocopherol transfer protein (ATTP) null mice (ATTP-/-) have a systemic deficiency of alpha-tocopherol (AT). The heart AT levels of ATTP-/- are <10% of those in ATTP+/+ mice. The genomic responses of heart to AT deficiency were determined in 3 months old male ATTP-/- mice and compared with their ATTP+/+ littermate controls using Affymetrix 430A 2.0 high density oligonucleotide arrays. Differential analysis of ∼13 000 genes identified repression of genes related to immune system and activation of genes related to lipid metabolism and inflammation with no significant change in the expression of classical antioxidant genes (catalase, superoxide dismutase, glutathione peroxidase) in ATTP-/- as compared to ATTP+/+ mice. The present data identifies novel classes of AT sensitive genes in heart tissue.

AB - Alpha-tocopherol transfer protein (ATTP) null mice (ATTP-/-) have a systemic deficiency of alpha-tocopherol (AT). The heart AT levels of ATTP-/- are <10% of those in ATTP+/+ mice. The genomic responses of heart to AT deficiency were determined in 3 months old male ATTP-/- mice and compared with their ATTP+/+ littermate controls using Affymetrix 430A 2.0 high density oligonucleotide arrays. Differential analysis of ∼13 000 genes identified repression of genes related to immune system and activation of genes related to lipid metabolism and inflammation with no significant change in the expression of classical antioxidant genes (catalase, superoxide dismutase, glutathione peroxidase) in ATTP-/- as compared to ATTP+/+ mice. The present data identifies novel classes of AT sensitive genes in heart tissue.

KW - Alpha-tocopherol

KW - Alpha-tocopherol transfer protein null mice

KW - Gene expression profiling

KW - Heart

UR - http://www.scopus.com/inward/record.url?scp=34047270886&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34047270886&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2007.03.017

DO - 10.1016/j.febslet.2007.03.017

M3 - Article

C2 - 17382327

AN - SCOPUS:34047270886

VL - 581

SP - 1572

EP - 1578

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 8

ER -