Abstract
α-tocopherol (α-T) may affect biological processes by modulating mRNA concentrations. This study screened the responses of ∼15,000 lung mRNAs to dietary α-T in mice. The lung was chosen as the target organ because it is subjected to cyclical variations in oxidant and inflammatory stressors and α-T has been implicated in their modulations. The analysis identified ∼400 mRNAs sensitive to α-T status of lungs determined by dietary α-T. The female lung transcriptome appears to be more sensitive to the α-T status than that of the male lungs. Here, we focus on the induction of 13 cytoskeleton genes by dietary α-T because they were similarly induced in the male and the female lungs. Their inductions were confirmed by quantitative-real-time-polymerase chain reaction (qRT-PCR). Immunohistochemical analyses of three of the encoded proteins suggest that they are expressed in lung vasculature and alveolar regions. The data suggest that the lung αT status may modulate cytoarchitecture of lungs.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 98-109 |
| Number of pages | 12 |
| Journal | Free Radical Research |
| Volume | 41 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2007 |
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Keywords
- Cytoskeleton
- Microarrays
- Oligonucleotide-arrays
- Smooth muscle
- Tocopherol
- Vitamin E
ASJC Scopus subject areas
- Biochemistry
Cite this
Genome wide responses of murine lungs to dietary α-tocopherol. / Oommen, Saji; Vasu, Vihas T.; Leonard, Scott W.; Traber, Maret G.; Cross, Carroll E; Gohil, Kishorchandra.
In: Free Radical Research, Vol. 41, No. 1, 01.2007, p. 98-109.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genome wide responses of murine lungs to dietary α-tocopherol
AU - Oommen, Saji
AU - Vasu, Vihas T.
AU - Leonard, Scott W.
AU - Traber, Maret G.
AU - Cross, Carroll E
AU - Gohil, Kishorchandra
PY - 2007/1
Y1 - 2007/1
N2 - α-tocopherol (α-T) may affect biological processes by modulating mRNA concentrations. This study screened the responses of ∼15,000 lung mRNAs to dietary α-T in mice. The lung was chosen as the target organ because it is subjected to cyclical variations in oxidant and inflammatory stressors and α-T has been implicated in their modulations. The analysis identified ∼400 mRNAs sensitive to α-T status of lungs determined by dietary α-T. The female lung transcriptome appears to be more sensitive to the α-T status than that of the male lungs. Here, we focus on the induction of 13 cytoskeleton genes by dietary α-T because they were similarly induced in the male and the female lungs. Their inductions were confirmed by quantitative-real-time-polymerase chain reaction (qRT-PCR). Immunohistochemical analyses of three of the encoded proteins suggest that they are expressed in lung vasculature and alveolar regions. The data suggest that the lung αT status may modulate cytoarchitecture of lungs.
AB - α-tocopherol (α-T) may affect biological processes by modulating mRNA concentrations. This study screened the responses of ∼15,000 lung mRNAs to dietary α-T in mice. The lung was chosen as the target organ because it is subjected to cyclical variations in oxidant and inflammatory stressors and α-T has been implicated in their modulations. The analysis identified ∼400 mRNAs sensitive to α-T status of lungs determined by dietary α-T. The female lung transcriptome appears to be more sensitive to the α-T status than that of the male lungs. Here, we focus on the induction of 13 cytoskeleton genes by dietary α-T because they were similarly induced in the male and the female lungs. Their inductions were confirmed by quantitative-real-time-polymerase chain reaction (qRT-PCR). Immunohistochemical analyses of three of the encoded proteins suggest that they are expressed in lung vasculature and alveolar regions. The data suggest that the lung αT status may modulate cytoarchitecture of lungs.
KW - Cytoskeleton
KW - Microarrays
KW - Oligonucleotide-arrays
KW - Smooth muscle
KW - Tocopherol
KW - Vitamin E
UR - http://www.scopus.com/inward/record.url?scp=33845443901&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845443901&partnerID=8YFLogxK
U2 - 10.1080/10715760600935567
DO - 10.1080/10715760600935567
M3 - Article
C2 - 17164183
AN - SCOPUS:33845443901
VL - 41
SP - 98
EP - 109
JO - Free Radical Research
JF - Free Radical Research
SN - 1071-5762
IS - 1
ER -