Abstract
Endogenous retroviruses (ERVs) constitute approximately 8-10% of the human and mouse genome. Some autoimmune diseases are attributed to the altered expression of ERVs. In this study, we examined the ERV expression profiles in lymphoid tissues and analyzed their biological properties. Tissues (spleen, thymus, and lymph nodes [axillary, inguinal, and mesenteric]) from C57BL/6J mice were analyzed for differential murine ERV (MuERV) expression by RT-PCR examination of polymorphic U3 sequences. Each tissue had a unique profile of MuERV expression. A genomic map identifying 60 putative MuERVs was established using 22 unique U3s as probes and their biological properties (primer binding site, coding potential, transcription regulatory element, tropism, recombination event, and integration age) were characterized. Interestingly, 12 putative MuERVs retained intact coding potentials for all three polypeptides essential for virus assembly and replication. We suggest that MuERV expression is differentially regulated in conjunction with the transcriptional environment of individual lymphoid tissues.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 263-273 |
| Number of pages | 11 |
| Journal | Virology |
| Volume | 373 |
| Issue number | 2 |
| DOIs | |
| State | Published - Apr 10 2008 |
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Keywords
- Coding potential
- Endogenous retrovirus
- Genome
- Lymph node
- Spleen
- Thymus
- Transcription
ASJC Scopus subject areas
- Virology
- Infectious Diseases
Cite this
Genome-wide expression profiles of endogenous retroviruses in lymphoid tissues and their biological properties. / Lee, Young Kwan; Chew, Alex; Phan, Ho H; Greenhalgh, David G; Cho, Kiho.
In: Virology, Vol. 373, No. 2, 10.04.2008, p. 263-273.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genome-wide expression profiles of endogenous retroviruses in lymphoid tissues and their biological properties
AU - Lee, Young Kwan
AU - Chew, Alex
AU - Phan, Ho H
AU - Greenhalgh, David G
AU - Cho, Kiho
PY - 2008/4/10
Y1 - 2008/4/10
N2 - Endogenous retroviruses (ERVs) constitute approximately 8-10% of the human and mouse genome. Some autoimmune diseases are attributed to the altered expression of ERVs. In this study, we examined the ERV expression profiles in lymphoid tissues and analyzed their biological properties. Tissues (spleen, thymus, and lymph nodes [axillary, inguinal, and mesenteric]) from C57BL/6J mice were analyzed for differential murine ERV (MuERV) expression by RT-PCR examination of polymorphic U3 sequences. Each tissue had a unique profile of MuERV expression. A genomic map identifying 60 putative MuERVs was established using 22 unique U3s as probes and their biological properties (primer binding site, coding potential, transcription regulatory element, tropism, recombination event, and integration age) were characterized. Interestingly, 12 putative MuERVs retained intact coding potentials for all three polypeptides essential for virus assembly and replication. We suggest that MuERV expression is differentially regulated in conjunction with the transcriptional environment of individual lymphoid tissues.
AB - Endogenous retroviruses (ERVs) constitute approximately 8-10% of the human and mouse genome. Some autoimmune diseases are attributed to the altered expression of ERVs. In this study, we examined the ERV expression profiles in lymphoid tissues and analyzed their biological properties. Tissues (spleen, thymus, and lymph nodes [axillary, inguinal, and mesenteric]) from C57BL/6J mice were analyzed for differential murine ERV (MuERV) expression by RT-PCR examination of polymorphic U3 sequences. Each tissue had a unique profile of MuERV expression. A genomic map identifying 60 putative MuERVs was established using 22 unique U3s as probes and their biological properties (primer binding site, coding potential, transcription regulatory element, tropism, recombination event, and integration age) were characterized. Interestingly, 12 putative MuERVs retained intact coding potentials for all three polypeptides essential for virus assembly and replication. We suggest that MuERV expression is differentially regulated in conjunction with the transcriptional environment of individual lymphoid tissues.
KW - Coding potential
KW - Endogenous retrovirus
KW - Genome
KW - Lymph node
KW - Spleen
KW - Thymus
KW - Transcription
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UR - http://www.scopus.com/inward/citedby.url?scp=40849137684&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2007.10.043
DO - 10.1016/j.virol.2007.10.043
M3 - Article
C2 - 18187179
AN - SCOPUS:40849137684
VL - 373
SP - 263
EP - 273
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
ER -