Genome-wide architecture of disease resistance genes in lettuce

Marilena Christopoulou, Sebastian Reyes Chin Wo, Alex Kozik, Leah K. McHale, Maria Jose Truco, Tadeusz Wroblewski, Richard W Michelmore

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Genome-wide motif searches identified 1134 genes in the lettuce reference genome of cv. Salinas that are potentially involved in pathogen recognition, of which 385 were predicted to encode nucleotide binding-leucine rich repeat receptor (NLR) proteins. Using a maximum-likelihood approach, we grouped the NLRs into 25 multigene families and 17 singletons. Forty-one percent of these NLR-encoding genes belong to three families, the largest being RGC16 with 62 genes in cv. Salinas. The majority of NLRencoding genes are located in five major resistance clusters (MRCs) on chromosomes 1, 2, 3, 4, and 8 and cosegregate with multiple disease resistance phenotypes. Most MRCs contain primarily members of a single NLR gene family but a few are more complex. MRC2 spans 73 Mb and contains 61 NLRs of six different gene families that cosegregate with nine disease resistance phenotypes. MRC3, which is 25 Mb, contains 22 RGC21 genes and colocates with Dm13. A library of 33 transgenic RNA interference tester stocks was generated for functional analysis of NLR-encoding genes that cosegregated with disease resistance phenotypes in each of the MRCs. Members of four NLR-encoding families, RGC1, RGC2, RGC21, and RGC12 were shown to be required for 16 disease resistance phenotypes in lettuce. The general composition of MRCs is conserved across different genotypes; however, the specific repertoire of NLRencoding genes varied particularly of the rapidly evolving Type I genes. These tester stocks are valuable resources for future analyses of additional resistance phenotypes.

Original languageEnglish (US)
Pages (from-to)2655-2669
Number of pages15
JournalG3: Genes, Genomes, Genetics
Volume5
Issue number12
DOIs
StatePublished - 2015

Fingerprint

Lettuce
Disease Resistance
Genome
Genes
Phenotype
Chromosomes, Human, Pair 2
Chromosomes, Human, Pair 1
Multigene Family
RNA Interference
Leucine
Libraries
Nucleotides
Genotype

Keywords

  • Bremia lactucae
  • Gene silencing
  • Lettuce downy mildew
  • NB-LRR
  • Reverse genetics

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Genetics(clinical)

Cite this

Christopoulou, M., Wo, S. R. C., Kozik, A., McHale, L. K., Truco, M. J., Wroblewski, T., & Michelmore, R. W. (2015). Genome-wide architecture of disease resistance genes in lettuce. G3: Genes, Genomes, Genetics, 5(12), 2655-2669. https://doi.org/10.1534/g3.115.020818

Genome-wide architecture of disease resistance genes in lettuce. / Christopoulou, Marilena; Wo, Sebastian Reyes Chin; Kozik, Alex; McHale, Leah K.; Truco, Maria Jose; Wroblewski, Tadeusz; Michelmore, Richard W.

In: G3: Genes, Genomes, Genetics, Vol. 5, No. 12, 2015, p. 2655-2669.

Research output: Contribution to journalArticle

Christopoulou, M, Wo, SRC, Kozik, A, McHale, LK, Truco, MJ, Wroblewski, T & Michelmore, RW 2015, 'Genome-wide architecture of disease resistance genes in lettuce', G3: Genes, Genomes, Genetics, vol. 5, no. 12, pp. 2655-2669. https://doi.org/10.1534/g3.115.020818
Christopoulou M, Wo SRC, Kozik A, McHale LK, Truco MJ, Wroblewski T et al. Genome-wide architecture of disease resistance genes in lettuce. G3: Genes, Genomes, Genetics. 2015;5(12):2655-2669. https://doi.org/10.1534/g3.115.020818
Christopoulou, Marilena ; Wo, Sebastian Reyes Chin ; Kozik, Alex ; McHale, Leah K. ; Truco, Maria Jose ; Wroblewski, Tadeusz ; Michelmore, Richard W. / Genome-wide architecture of disease resistance genes in lettuce. In: G3: Genes, Genomes, Genetics. 2015 ; Vol. 5, No. 12. pp. 2655-2669.
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