Genome-wide analysis of transcription factor-binding sites in skeletal muscle cells using ChIP-seq

Chung Il An, Nobuko Hagiwara

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Transcriptional regulation of gene expression constitutes a fundamental mechanism of many developmental processes. Therefore, identification and characterization of binding sites of transcription factors are important for uncovering the mechanisms of a particular developmental process. Here, we describe detailed procedures for genome-wide analysis of binding sites of a transcription factor involved in the fiber-type differentiation of skeletal muscle. By conducting ChIP-seq followed by a series of downstream analyses, in-depth information on binding sites of transcription factors can be obtained in a genome-wide manner.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages51-64
Number of pages14
Volume1067
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1067
ISSN (Print)10643745

Keywords

  • Alignment
  • ChIP-seq
  • Library preparation
  • Motif discovery
  • Peak calling
  • Primary myotubes
  • Skeletal muscle differentiation
  • Sox6

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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