Genome-wide analysis of chromosomal features repressing human immunodeficiency virus transcription

M. K. Lewinski, D. Bisgrove, P. Shinn, Hongwu Chen, C. Hoffmann, S. Hannenhalli, E. Verdin, C. C. Berry, J. R. Ecker, F. D. Bushman

Research output: Contribution to journalArticle

201 Citations (Scopus)

Abstract

We have investigated regulatory sequences in noncoding human DNA that are associated with repression of an integrated human immunodeficiency virus type 1 (HIV-1) promoter. HIV-1 integration results in the formation of precise and homogeneous junctions between viral and host DNA, but integration takes place at many locations. Thus, the variation in HIV-1 gene expression at different integration sites reports the activity of regulatory sequences at nearby chromosomal positions. Negative regulation of HIV transcription is of particular interest because of its association with maintaining HIV in a latent state in cells from infected patients. To identify chromosomal regulators of HIV transcription, we infected Jurkat T cells with an HIV-based vector transducing green fluorescent protein (GFP) and separated cells into populations containing well-expressed (GFP-positive) or poorly expressed (GFP-negative) proviruses. We then determined the chromosomal locations of the two classes by sequencing 971 junctions between viral and cellular DNA. Possible effects of endogenous cellular transcription were characterized by transcriptional profiling. Low-level GFP expression correlated with integration in (i) gene deserts, (ii) centromeric heterochromatin, and (iii) very highly expressed cellular genes. These data provide a genome-wide picture of chromosomal features that repress transcription and suggest models for transcriptional latency in cells from HIV-infected patients.

Original languageEnglish (US)
Pages (from-to)6610-6619
Number of pages10
JournalJournal of Virology
Volume79
Issue number11
DOIs
StatePublished - Jun 2005
Externally publishedYes

Fingerprint

Human immunodeficiency virus
green fluorescent protein
Green Fluorescent Proteins
transcription (genetics)
Human immunodeficiency virus 1
HIV
Genome
regulatory sequences
genome
HIV-1
Viral DNA
DNA
proviruses
Virus Integration
heterochromatin
cells
Proviruses
Jurkat Cells
Heterochromatin
deserts

ASJC Scopus subject areas

  • Immunology

Cite this

Lewinski, M. K., Bisgrove, D., Shinn, P., Chen, H., Hoffmann, C., Hannenhalli, S., ... Bushman, F. D. (2005). Genome-wide analysis of chromosomal features repressing human immunodeficiency virus transcription. Journal of Virology, 79(11), 6610-6619. https://doi.org/10.1128/JVI.79.11.6610-6619.2005

Genome-wide analysis of chromosomal features repressing human immunodeficiency virus transcription. / Lewinski, M. K.; Bisgrove, D.; Shinn, P.; Chen, Hongwu; Hoffmann, C.; Hannenhalli, S.; Verdin, E.; Berry, C. C.; Ecker, J. R.; Bushman, F. D.

In: Journal of Virology, Vol. 79, No. 11, 06.2005, p. 6610-6619.

Research output: Contribution to journalArticle

Lewinski, MK, Bisgrove, D, Shinn, P, Chen, H, Hoffmann, C, Hannenhalli, S, Verdin, E, Berry, CC, Ecker, JR & Bushman, FD 2005, 'Genome-wide analysis of chromosomal features repressing human immunodeficiency virus transcription', Journal of Virology, vol. 79, no. 11, pp. 6610-6619. https://doi.org/10.1128/JVI.79.11.6610-6619.2005
Lewinski, M. K. ; Bisgrove, D. ; Shinn, P. ; Chen, Hongwu ; Hoffmann, C. ; Hannenhalli, S. ; Verdin, E. ; Berry, C. C. ; Ecker, J. R. ; Bushman, F. D. / Genome-wide analysis of chromosomal features repressing human immunodeficiency virus transcription. In: Journal of Virology. 2005 ; Vol. 79, No. 11. pp. 6610-6619.
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