Abstract
Genomic DNA of the myxobacterium Myxococcus xanthus was digested with the rare cutting restriction endonuclease AseI or SpeI, and the restriction products wre separated by pulsed-field gel electrophoresis. Transposons Tn5-132 and Tn5 lac, which contain AseI restriction sites, were used to determine the number of restriction fragments in each band. The size of the genome was determined by adding the molecular sizes of the restriction products. The genomes of strains DK101, MD2, and DZF1 had identical restriction patterns and were estimated to be 9,454 ± 101 kilobase pairs from the AseI digestions and 9,453 ± 106 kilobase pairs from the SpeI digestions. DK1622, which was derived from DK101 by treatment with UV light, has suffered a 220- to 222-kilobase-pair deletion that removed an AseI and an SpeI restriction site. The deleted DNA may consist exclusively of Mx alpha-associated sequences.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4206-4213 |
| Number of pages | 8 |
| Journal | Journal of Bacteriology |
| Volume | 172 |
| Issue number | 8 |
| DOIs | |
| State | Published - Jan 1 1990 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Microbiology
- Molecular Biology
Cite this
Genome size of Myxococcus xanthus determined by pulsed-field gel electrophoresis. / Chen, Hongwu; Keseler, I. M.; Shimkets, L. J.
In: Journal of Bacteriology, Vol. 172, No. 8, 01.01.1990, p. 4206-4213.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genome size of Myxococcus xanthus determined by pulsed-field gel electrophoresis
AU - Chen, Hongwu
AU - Keseler, I. M.
AU - Shimkets, L. J.
PY - 1990/1/1
Y1 - 1990/1/1
N2 - Genomic DNA of the myxobacterium Myxococcus xanthus was digested with the rare cutting restriction endonuclease AseI or SpeI, and the restriction products wre separated by pulsed-field gel electrophoresis. Transposons Tn5-132 and Tn5 lac, which contain AseI restriction sites, were used to determine the number of restriction fragments in each band. The size of the genome was determined by adding the molecular sizes of the restriction products. The genomes of strains DK101, MD2, and DZF1 had identical restriction patterns and were estimated to be 9,454 ± 101 kilobase pairs from the AseI digestions and 9,453 ± 106 kilobase pairs from the SpeI digestions. DK1622, which was derived from DK101 by treatment with UV light, has suffered a 220- to 222-kilobase-pair deletion that removed an AseI and an SpeI restriction site. The deleted DNA may consist exclusively of Mx alpha-associated sequences.
AB - Genomic DNA of the myxobacterium Myxococcus xanthus was digested with the rare cutting restriction endonuclease AseI or SpeI, and the restriction products wre separated by pulsed-field gel electrophoresis. Transposons Tn5-132 and Tn5 lac, which contain AseI restriction sites, were used to determine the number of restriction fragments in each band. The size of the genome was determined by adding the molecular sizes of the restriction products. The genomes of strains DK101, MD2, and DZF1 had identical restriction patterns and were estimated to be 9,454 ± 101 kilobase pairs from the AseI digestions and 9,453 ± 106 kilobase pairs from the SpeI digestions. DK1622, which was derived from DK101 by treatment with UV light, has suffered a 220- to 222-kilobase-pair deletion that removed an AseI and an SpeI restriction site. The deleted DNA may consist exclusively of Mx alpha-associated sequences.
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U2 - 10.1128/jb.172.8.4206-4213.1990
DO - 10.1128/jb.172.8.4206-4213.1990
M3 - Article
C2 - 2165472
AN - SCOPUS:0025324973
VL - 172
SP - 4206
EP - 4213
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 8
ER -