Genome size and complexity of the obligate fungal pathogen, Bremia lactucae

David M. Francis; Scot H. Hulbert; Richard W Michelmore

doi:10.1016/0147-5975(90)90053-V

Genome size and complexity of the obligate fungal pathogen, Bremia lactucae

David M. Francis, Scot H. Hulbert, Richard W Michelmore

Medical Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

The size and organization of the genome of Bremia lactucae, a highly specialized fungal pathogen of lettuce, has been characterized using dot blot genomic reconstructions, reverse genomic blots, and genomic DNA reassociation kinetics. The haploid genome contains 5 × 10⁷ bp of DNA and 65% of the nuclear DNA is repeated. Low copy sequences are interspersed with repeated sequences in a short-period interspersion pattern. This pattern of genome organization is different to that described for other fungi. Although most fungi have been shown to contain some form of repetitive DNA other than the ribosomal repeat, the high percentage of repetitive DNA and the interspersion of low copy and repeated sequences are atypical of fungi characterized previously.

Original language	English (US)
Pages (from-to)	299-309
Number of pages	11
Journal	Experimental Mycology
Volume	14
Issue number	4
DOIs	https://doi.org/10.1016/0147-5975(90)90053-V
State	Published - 1990

Keywords

Bremia lactucae
gene-for-gene
genome complexity
genome size
Lactuca sativa
lettuce
Oomycete
repetitive DNA

ASJC Scopus subject areas

Applied Microbiology and Biotechnology

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10.1016/0147-5975(90)90053-V

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abstract = "The size and organization of the genome of Bremia lactucae, a highly specialized fungal pathogen of lettuce, has been characterized using dot blot genomic reconstructions, reverse genomic blots, and genomic DNA reassociation kinetics. The haploid genome contains 5 × 107 bp of DNA and 65% of the nuclear DNA is repeated. Low copy sequences are interspersed with repeated sequences in a short-period interspersion pattern. This pattern of genome organization is different to that described for other fungi. Although most fungi have been shown to contain some form of repetitive DNA other than the ribosomal repeat, the high percentage of repetitive DNA and the interspersion of low copy and repeated sequences are atypical of fungi characterized previously.",

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AU - Francis, David M.

AU - Hulbert, Scot H.

AU - Michelmore, Richard W

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N2 - The size and organization of the genome of Bremia lactucae, a highly specialized fungal pathogen of lettuce, has been characterized using dot blot genomic reconstructions, reverse genomic blots, and genomic DNA reassociation kinetics. The haploid genome contains 5 × 107 bp of DNA and 65% of the nuclear DNA is repeated. Low copy sequences are interspersed with repeated sequences in a short-period interspersion pattern. This pattern of genome organization is different to that described for other fungi. Although most fungi have been shown to contain some form of repetitive DNA other than the ribosomal repeat, the high percentage of repetitive DNA and the interspersion of low copy and repeated sequences are atypical of fungi characterized previously.

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KW - Bremia lactucae

KW - gene-for-gene

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KW - Lactuca sativa

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KW - Oomycete

KW - repetitive DNA

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Genome size and complexity of the obligate fungal pathogen, Bremia lactucae

Abstract

Keywords

ASJC Scopus subject areas

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