Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis

John F. Heidelberg, Ian T. Paulsen, Karen E. Nelson, Eric J. Gaidos, William C. Nelson, Timothy D. Read, Jonathan A Eisen, Rekha Seshadri, Naomi Ward, Barbara Methe, Rebecca A. Clayton, Terry Meyer, Alexandre Tsapin, James Scott, Maureen Beanan, Lauren Brinkac, Sean Daugherty, Robert T. DeBoy, Robert J. Dodson, A. Scott DurkinDaniel H. Haft, James F. Kolonay, Ramana Madupu, Jeremy D. Peterson, Lowell A. Umayam, Owen White, Alex M. Wolf, Jessica Vamathevan, Janice Weidman, Marjorie Impraim, Kathy Lee, Kristy Berry, Chris Lee, Jacob Mueller, Hoda Khouri, John Gill, Terry R. Utterback, Lisa A. McDonald, Tamara V. Feldblyum, Hamilton O. Smith, J. Craig Venter, Kenneth H. Nealson, Claire M. Fraser

Research output: Contribution to journalArticle

572 Scopus citations

Abstract

Shewanella oneidensis is an important model organism for bioremediation studies because of its diverse respiratory capabilities, conferred in part by multicomponent, branched electron transport systems. Here we report the sequencing of the S. oneidensis genome, which consists of a 4,969,803-base pair circular chromosome with 4,758 predicted protein-encoding open reading frames (CDS) and a 161,613-base pair plasmid with 173 CDSs. We identified the first Shewanella lambda-like phage, providing a potential tool for further genome engineering. Genome analysis revealed 39 c-type cytochromes, including 32 previously unidentified in S. oneidensis, and a novel periplasmic [Fe] hydrogenase, which are integral members of the electron transport system. This genome sequence represents a critical step in the elucidation of the pathways for reduction (and bioremediation) of pollutants such as uranium (U) and chromium (Cr), and offers a starting point for defining this organism's complex electron transport systems and metal ion-reducing capabilities.

Original languageEnglish (US)
Pages (from-to)1118-1123
Number of pages6
JournalNature Biotechnology
Volume20
Issue number11
DOIs
StatePublished - Nov 1 2002
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology

Fingerprint Dive into the research topics of 'Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis'. Together they form a unique fingerprint.

  • Cite this

    Heidelberg, J. F., Paulsen, I. T., Nelson, K. E., Gaidos, E. J., Nelson, W. C., Read, T. D., Eisen, J. A., Seshadri, R., Ward, N., Methe, B., Clayton, R. A., Meyer, T., Tsapin, A., Scott, J., Beanan, M., Brinkac, L., Daugherty, S., DeBoy, R. T., Dodson, R. J., ... Fraser, C. M. (2002). Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis. Nature Biotechnology, 20(11), 1118-1123. https://doi.org/10.1038/nbt749