Genome report: Identification and validation of antigenic proteins from Pajaroellobacter abortibovis using de novo genome sequence assembly and reverse vaccinology

Bryan T. Welly, Michael R. Miller, Jeffrey L Stott, Myra Blanchard, Alma D. Islas-Trejo, Sean M. O'Rourke, Amy E. Young, Juan F. Medrano, Alison L. Van Eenennaam

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Epizootic bovine abortion (EBA), or "foothill abortion," is the leading cause of beef cattle abortion in California and has also been reported in Nevada and Oregon. In the 1970s, the soft-shelled tick Ornithodoros coriaceus, or "pajaroello tick," was confirmed as the disease-transmitting vector. In 2005, a novel Deltaproteobacterium was discovered as the etiologic agent of EBA (aoEBA), recently named Pajaroellobacter abortibovis. This organism cannot be grown in culture using traditional microbiological techniques; it can only be grown in experimentally-infected severe combined immunodeficient (SCID) mice. The objectives of this study were to perform a de novo genome assembly for P. abortibovis and identify and validate potential antigenic proteins as candidates for future recombinant vaccine development. DNA and RNA were extracted from spleen tissue collected from experimentally-infected SCID mice following exposure to P. abortibovis. This combination of mouse and bacterial DNA was sequenced and aligned to the mouse genome. Mouse sequences were subtracted from the sequence pool and the remaining sequences were de novo assembled at 50x coverage into a 1.82 Mbp complete closed circular Deltaproteobacterial genome containing 2250 putative protein-coding sequences. Phylogenetic analysis of P. abortibovis predicts that this bacterium is most closely related to the organisms of the order Myxococcales, referred to as Myxobacteria. In silico prediction of vaccine candidates was performed using a reverse vaccinology approach resulting in the identification and ranking of the top 10 candidate proteins that are likely to be antigenic. Immunologic testing of these candidate proteins confirmed antigenicity of seven of the nine expressed protein candidates using serum from P. abortibovis immunized mice.

Original languageEnglish (US)
Pages (from-to)321-331
Number of pages11
JournalG3: Genes, Genomes, Genetics
Volume7
Issue number2
DOIs
StatePublished - 2017

Fingerprint

Genome
Myxococcales
SCID Mice
Proteins
Argasidae
Deltaproteobacteria
Ornithodoros
Microbiological Techniques
Disease Vectors
Bacterial DNA
Synthetic Vaccines
Ticks
Computer Simulation
Vaccines
Spleen
RNA
Bacteria
DNA
Serum

Keywords

  • Epizootic bovine abortion
  • Ornithodoros coriaceus
  • Pajaroellobacter abortibovis
  • Recombinant vaccine
  • Reverse vaccinology

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Genome report : Identification and validation of antigenic proteins from Pajaroellobacter abortibovis using de novo genome sequence assembly and reverse vaccinology. / Welly, Bryan T.; Miller, Michael R.; Stott, Jeffrey L; Blanchard, Myra; Islas-Trejo, Alma D.; O'Rourke, Sean M.; Young, Amy E.; Medrano, Juan F.; Van Eenennaam, Alison L.

In: G3: Genes, Genomes, Genetics, Vol. 7, No. 2, 2017, p. 321-331.

Research output: Contribution to journalArticle

Welly, Bryan T. ; Miller, Michael R. ; Stott, Jeffrey L ; Blanchard, Myra ; Islas-Trejo, Alma D. ; O'Rourke, Sean M. ; Young, Amy E. ; Medrano, Juan F. ; Van Eenennaam, Alison L. / Genome report : Identification and validation of antigenic proteins from Pajaroellobacter abortibovis using de novo genome sequence assembly and reverse vaccinology. In: G3: Genes, Genomes, Genetics. 2017 ; Vol. 7, No. 2. pp. 321-331.
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