Genome-based identification and characterization of a putative mucin-binding protein from the surface of Streptococcus pneumoniae

Daniela Bumbaca, James E Littlejohn, Hannah Nayakanti, Alexander H. Lucas, Daniel J. Rigden, Michael Y. Galperin, Mark J. Jedrzejas

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Streptococcus pneumoniae open reading frame SP1492 encodes a surface protein that contains a novel conserved domain similar to the repeated fragments of mucin-binding proteins from lactobacilli and lactococci. To investigate the functional role(s) of this protein and its potential adhesive properties, the surface-exposed region of SP1492 was expressed in Escherichia coli, purified to homogeneity, and partially characterized by biophysical and immunological methods. Circular dichroism and sedimentation measurements confirmed that SP1492 is an all-β protein that exists in solution as a monomer. The SP1492 protein has been shown to be expressed by S. pneumoniae and was experimentally localized to its surface. The protein functional domain binds to mucins II and III from porcine stomach and to purified submaxillary bovine gland mucin. It appears to be one of the very few unambiguous pneumococcal adhesin molecules known to date. A hypothetical model constructed by ab initio techniques predicts a novel β-sandwich protein structure.

Original languageEnglish (US)
Pages (from-to)547-558
Number of pages12
JournalProteins: Structure, Function and Genetics
Volume66
Issue number3
DOIs
StatePublished - Feb 15 2007
Externally publishedYes

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Keywords

  • Adhesin
  • Carbohydrate
  • Gram-positive bacteria
  • Mucin
  • Mucin-binding protein
  • Streptococcus pneumoniae

ASJC Scopus subject areas

  • Genetics
  • Structural Biology
  • Biochemistry

Cite this

Bumbaca, D., Littlejohn, J. E., Nayakanti, H., Lucas, A. H., Rigden, D. J., Galperin, M. Y., & Jedrzejas, M. J. (2007). Genome-based identification and characterization of a putative mucin-binding protein from the surface of Streptococcus pneumoniae. Proteins: Structure, Function and Genetics, 66(3), 547-558. https://doi.org/10.1002/prot.21205