Abstract
Newcastle disease virus (NDV) is a highly contagious avian pathogen that poses a tremendous threat to poultry producers in endemic zones due to its epidemic potential. To investigate host genetic resistance to NDV while under the effects of heat stress, a genome-wide association study (GWAS) was performed on Hy-Line Brown layer chickens that were challenged with NDV while under high ambient temperature to identify regions associated with host viral titer, circulating anti-NDV antibody titer, and body weight change. A single nucleotide polymorphism (SNP) on chromosome 1 was associated with viral titer at two days post-infection (dpi), while 30 SNPs spanning a quantitative trait loci (QTL) on chromosome 24 were associated with viral titer at 6 dpi. Immune related genes, such as CAMK1d and CCDC3 on chromosome 1, associated with viral titer at 2 dpi, and TIRAP, ETS1, and KIRREL3, associated with viral titer at 6 dpi, were located in two QTL regions for viral titer that were identified in this study. This study identified genomic regions and candidate genes that are associated with response to NDV during heat stress in Hy-Line Brown layer chickens. Regions identified for viral titer on chromosome 1 and 24, at 2 and 6 dpi, respectively, included several genes that have key roles in regulating the immune response.
| Original language | English (US) |
|---|---|
| Article number | 61 |
| Journal | Genes |
| Volume | 10 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2019 |
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Keywords
- Chicken
- GWAS
- Heat stress
- NDV
- QTL
ASJC Scopus subject areas
- Genetics
- Genetics(clinical)
Cite this
Genetics and genomic regions affecting response to newcastle disease virus infection under heat stress in layer chickens. / Saelao, Perot; Wang, Ying; Chanthavixay, Ganrea; Gallardo, Rodrigo A; Wolc, Anna; Dekkers, Jack C.M.; Lamont, Susan J.; Kelly, Terra; Zhou, Huaijun.
In: Genes, Vol. 10, No. 1, 61, 01.01.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genetics and genomic regions affecting response to newcastle disease virus infection under heat stress in layer chickens
AU - Saelao, Perot
AU - Wang, Ying
AU - Chanthavixay, Ganrea
AU - Gallardo, Rodrigo A
AU - Wolc, Anna
AU - Dekkers, Jack C.M.
AU - Lamont, Susan J.
AU - Kelly, Terra
AU - Zhou, Huaijun
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Newcastle disease virus (NDV) is a highly contagious avian pathogen that poses a tremendous threat to poultry producers in endemic zones due to its epidemic potential. To investigate host genetic resistance to NDV while under the effects of heat stress, a genome-wide association study (GWAS) was performed on Hy-Line Brown layer chickens that were challenged with NDV while under high ambient temperature to identify regions associated with host viral titer, circulating anti-NDV antibody titer, and body weight change. A single nucleotide polymorphism (SNP) on chromosome 1 was associated with viral titer at two days post-infection (dpi), while 30 SNPs spanning a quantitative trait loci (QTL) on chromosome 24 were associated with viral titer at 6 dpi. Immune related genes, such as CAMK1d and CCDC3 on chromosome 1, associated with viral titer at 2 dpi, and TIRAP, ETS1, and KIRREL3, associated with viral titer at 6 dpi, were located in two QTL regions for viral titer that were identified in this study. This study identified genomic regions and candidate genes that are associated with response to NDV during heat stress in Hy-Line Brown layer chickens. Regions identified for viral titer on chromosome 1 and 24, at 2 and 6 dpi, respectively, included several genes that have key roles in regulating the immune response.
AB - Newcastle disease virus (NDV) is a highly contagious avian pathogen that poses a tremendous threat to poultry producers in endemic zones due to its epidemic potential. To investigate host genetic resistance to NDV while under the effects of heat stress, a genome-wide association study (GWAS) was performed on Hy-Line Brown layer chickens that were challenged with NDV while under high ambient temperature to identify regions associated with host viral titer, circulating anti-NDV antibody titer, and body weight change. A single nucleotide polymorphism (SNP) on chromosome 1 was associated with viral titer at two days post-infection (dpi), while 30 SNPs spanning a quantitative trait loci (QTL) on chromosome 24 were associated with viral titer at 6 dpi. Immune related genes, such as CAMK1d and CCDC3 on chromosome 1, associated with viral titer at 2 dpi, and TIRAP, ETS1, and KIRREL3, associated with viral titer at 6 dpi, were located in two QTL regions for viral titer that were identified in this study. This study identified genomic regions and candidate genes that are associated with response to NDV during heat stress in Hy-Line Brown layer chickens. Regions identified for viral titer on chromosome 1 and 24, at 2 and 6 dpi, respectively, included several genes that have key roles in regulating the immune response.
KW - Chicken
KW - GWAS
KW - Heat stress
KW - NDV
KW - QTL
UR - http://www.scopus.com/inward/record.url?scp=85060778168&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85060778168&partnerID=8YFLogxK
U2 - 10.3390/genes10010061
DO - 10.3390/genes10010061
M3 - Article
AN - SCOPUS:85060778168
VL - 10
JO - Genes
JF - Genes
SN - 2073-4425
IS - 1
M1 - 61
ER -