Genetics and genomic regions affecting response to newcastle disease virus infection under heat stress in layer chickens

Perot Saelao, Ying Wang, Ganrea Chanthavixay, Rodrigo A Gallardo, Anna Wolc, Jack C.M. Dekkers, Susan J. Lamont, Terra Kelly, Huaijun Zhou

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Newcastle disease virus (NDV) is a highly contagious avian pathogen that poses a tremendous threat to poultry producers in endemic zones due to its epidemic potential. To investigate host genetic resistance to NDV while under the effects of heat stress, a genome-wide association study (GWAS) was performed on Hy-Line Brown layer chickens that were challenged with NDV while under high ambient temperature to identify regions associated with host viral titer, circulating anti-NDV antibody titer, and body weight change. A single nucleotide polymorphism (SNP) on chromosome 1 was associated with viral titer at two days post-infection (dpi), while 30 SNPs spanning a quantitative trait loci (QTL) on chromosome 24 were associated with viral titer at 6 dpi. Immune related genes, such as CAMK1d and CCDC3 on chromosome 1, associated with viral titer at 2 dpi, and TIRAP, ETS1, and KIRREL3, associated with viral titer at 6 dpi, were located in two QTL regions for viral titer that were identified in this study. This study identified genomic regions and candidate genes that are associated with response to NDV during heat stress in Hy-Line Brown layer chickens. Regions identified for viral titer on chromosome 1 and 24, at 2 and 6 dpi, respectively, included several genes that have key roles in regulating the immune response.

Original languageEnglish (US)
Article number61
JournalGenes
Volume10
Issue number1
DOIs
StatePublished - Jan 1 2019

Fingerprint

Newcastle disease virus
Virus Diseases
Chickens
Hot Temperature
Chromosomes, Human, Pair 1
Infection
Quantitative Trait Loci
Single Nucleotide Polymorphism
Genes
Body Weight Changes
Genome-Wide Association Study
Poultry
Viral Load
Chromosomes
Temperature
Antibodies

Keywords

  • Chicken
  • GWAS
  • Heat stress
  • NDV
  • QTL

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Genetics and genomic regions affecting response to newcastle disease virus infection under heat stress in layer chickens. / Saelao, Perot; Wang, Ying; Chanthavixay, Ganrea; Gallardo, Rodrigo A; Wolc, Anna; Dekkers, Jack C.M.; Lamont, Susan J.; Kelly, Terra; Zhou, Huaijun.

In: Genes, Vol. 10, No. 1, 61, 01.01.2019.

Research output: Contribution to journalArticle

Saelao, P, Wang, Y, Chanthavixay, G, Gallardo, RA, Wolc, A, Dekkers, JCM, Lamont, SJ, Kelly, T & Zhou, H 2019, 'Genetics and genomic regions affecting response to newcastle disease virus infection under heat stress in layer chickens', Genes, vol. 10, no. 1, 61. https://doi.org/10.3390/genes10010061
Saelao, Perot ; Wang, Ying ; Chanthavixay, Ganrea ; Gallardo, Rodrigo A ; Wolc, Anna ; Dekkers, Jack C.M. ; Lamont, Susan J. ; Kelly, Terra ; Zhou, Huaijun. / Genetics and genomic regions affecting response to newcastle disease virus infection under heat stress in layer chickens. In: Genes. 2019 ; Vol. 10, No. 1.
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