Genetically modified Chinese hamster ovary (CHO) cells for studying the genotoxicity of heterocyclic amines from cooked foods

Larry H. Thompson, Rebekah W. Wu, James S. Felton

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

We have developed metabolically competent Chinese hamster ovary (CHO) cells to evaluate the genotoxicity associated with heterocyclic amines, such as those that are present in cooked foods. Into repair-deficient UV5 cells we introduced cDNAs for expressing cytochrome P450IA2 and acetyltransferases. We then genetically reverted these transformed lines to obtain matched metabolically competent repair-deficient/proficient lines. For a high mutagenic response, we find a requirement for acetyltransferase with 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) but not with 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). This system allows for both quantifying mutagenesis and analyzing the mutational spectra produced by heterocyclic amines.

Original languageEnglish (US)
Pages (from-to)883-889
Number of pages7
JournalToxicology Letters
Volume82-83
Issue numberC
DOIs
StatePublished - 1995
Externally publishedYes

Keywords

  • DNA repair
  • Heterocyclic amines
  • IQ
  • Metabolic activation
  • Mutagenesis
  • PhIP

ASJC Scopus subject areas

  • Toxicology

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