Abstract
We have developed metabolically competent Chinese hamster ovary (CHO) cells to evaluate the genotoxicity associated with heterocyclic amines, such as those that are present in cooked foods. Into repair-deficient UV5 cells we introduced cDNAs for expressing cytochrome P450IA2 and acetyltransferases. We then genetically reverted these transformed lines to obtain matched metabolically competent repair-deficient/proficient lines. For a high mutagenic response, we find a requirement for acetyltransferase with 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) but not with 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). This system allows for both quantifying mutagenesis and analyzing the mutational spectra produced by heterocyclic amines.
Original language | English (US) |
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Pages (from-to) | 883-889 |
Number of pages | 7 |
Journal | Toxicology Letters |
Volume | 82-83 |
Issue number | C |
DOIs | |
State | Published - 1995 |
Externally published | Yes |
Keywords
- DNA repair
- Heterocyclic amines
- IQ
- Metabolic activation
- Mutagenesis
- PhIP
ASJC Scopus subject areas
- Toxicology