Abstract
We have developed metabolically competent Chinese hamster ovary (CHO) cells to evaluate the genotoxicity associated with heterocyclic amines, such as those that are present in cooked foods. Into repair-deficient UV5 cells we introduced cDNAs for expressing cytochrome P450IA2 and acetyltransferases. We then genetically reverted these transformed lines to obtain matched metabolically competent repair-deficient/proficient lines. For a high mutagenic response, we find a requirement for acetyltransferase with 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) but not with 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). This system allows for both quantifying mutagenesis and analyzing the mutational spectra produced by heterocyclic amines.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 883-889 |
| Number of pages | 7 |
| Journal | Toxicology Letters |
| Volume | 82-83 |
| Issue number | C |
| DOIs | |
| State | Published - 1995 |
| Externally published | Yes |
Fingerprint
Keywords
- DNA repair
- Heterocyclic amines
- IQ
- Metabolic activation
- Mutagenesis
- PhIP
ASJC Scopus subject areas
- Toxicology
Cite this
Genetically modified Chinese hamster ovary (CHO) cells for studying the genotoxicity of heterocyclic amines from cooked foods. / Thompson, Larry H.; Wu, Rebekah W.; Felton, James S.
In: Toxicology Letters, Vol. 82-83, No. C, 1995, p. 883-889.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genetically modified Chinese hamster ovary (CHO) cells for studying the genotoxicity of heterocyclic amines from cooked foods
AU - Thompson, Larry H.
AU - Wu, Rebekah W.
AU - Felton, James S.
PY - 1995
Y1 - 1995
N2 - We have developed metabolically competent Chinese hamster ovary (CHO) cells to evaluate the genotoxicity associated with heterocyclic amines, such as those that are present in cooked foods. Into repair-deficient UV5 cells we introduced cDNAs for expressing cytochrome P450IA2 and acetyltransferases. We then genetically reverted these transformed lines to obtain matched metabolically competent repair-deficient/proficient lines. For a high mutagenic response, we find a requirement for acetyltransferase with 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) but not with 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). This system allows for both quantifying mutagenesis and analyzing the mutational spectra produced by heterocyclic amines.
AB - We have developed metabolically competent Chinese hamster ovary (CHO) cells to evaluate the genotoxicity associated with heterocyclic amines, such as those that are present in cooked foods. Into repair-deficient UV5 cells we introduced cDNAs for expressing cytochrome P450IA2 and acetyltransferases. We then genetically reverted these transformed lines to obtain matched metabolically competent repair-deficient/proficient lines. For a high mutagenic response, we find a requirement for acetyltransferase with 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) but not with 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). This system allows for both quantifying mutagenesis and analyzing the mutational spectra produced by heterocyclic amines.
KW - DNA repair
KW - Heterocyclic amines
KW - IQ
KW - Metabolic activation
KW - Mutagenesis
KW - PhIP
UR - http://www.scopus.com/inward/record.url?scp=0029609893&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029609893&partnerID=8YFLogxK
U2 - 10.1016/0378-4274(95)03527-3
DO - 10.1016/0378-4274(95)03527-3
M3 - Article
C2 - 8597157
AN - SCOPUS:0029609893
VL - 82-83
SP - 883
EP - 889
JO - Toxicology Letters
JF - Toxicology Letters
SN - 0378-4274
IS - C
ER -