Abstract
Neurons and glia are functionally organized into circuits and higher order structures via synaptic connectivity, well-orchestrated molecular signaling and activity-dependent refinement. Such organization allows the precise information processing required for complex behaviors. Disruption of nervous systems by genetic deficiency or events such as trauma or environmental exposure can produce a diseased state in which certain aspects of inter-neuron signaling are impaired. Optical imaging techniques allow the direct visualization of individual neurons in a circuit environment. Imaging probes specific for given biomolecules might help elucidate their contribution to proper circuit function. Genetically encoded sensors can visualize trafficking of particular molecules in defined neuronal populations, non-invasively in intact brain or reduced preparations. Sensor analysis in healthy and diseased brains might reveal important differences and shed light on the development and progression of nervous system disorders. We review the field of genetically encoded sensors for molecules and cellular events and their potential applicability to the study of nervous system disease.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 27-35 |
| Number of pages | 9 |
| Journal | Drug Discovery Today: Disease Models |
| Volume | 5 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 2008 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Drug Discovery
- Molecular Medicine
Cite this
Genetically encoded fluorescent sensors for studying healthy and diseased nervous systems. / Tian, Lin; Looger, Loren L.
In: Drug Discovery Today: Disease Models, Vol. 5, No. 1, 03.2008, p. 27-35.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genetically encoded fluorescent sensors for studying healthy and diseased nervous systems
AU - Tian, Lin
AU - Looger, Loren L.
PY - 2008/3
Y1 - 2008/3
N2 - Neurons and glia are functionally organized into circuits and higher order structures via synaptic connectivity, well-orchestrated molecular signaling and activity-dependent refinement. Such organization allows the precise information processing required for complex behaviors. Disruption of nervous systems by genetic deficiency or events such as trauma or environmental exposure can produce a diseased state in which certain aspects of inter-neuron signaling are impaired. Optical imaging techniques allow the direct visualization of individual neurons in a circuit environment. Imaging probes specific for given biomolecules might help elucidate their contribution to proper circuit function. Genetically encoded sensors can visualize trafficking of particular molecules in defined neuronal populations, non-invasively in intact brain or reduced preparations. Sensor analysis in healthy and diseased brains might reveal important differences and shed light on the development and progression of nervous system disorders. We review the field of genetically encoded sensors for molecules and cellular events and their potential applicability to the study of nervous system disease.
AB - Neurons and glia are functionally organized into circuits and higher order structures via synaptic connectivity, well-orchestrated molecular signaling and activity-dependent refinement. Such organization allows the precise information processing required for complex behaviors. Disruption of nervous systems by genetic deficiency or events such as trauma or environmental exposure can produce a diseased state in which certain aspects of inter-neuron signaling are impaired. Optical imaging techniques allow the direct visualization of individual neurons in a circuit environment. Imaging probes specific for given biomolecules might help elucidate their contribution to proper circuit function. Genetically encoded sensors can visualize trafficking of particular molecules in defined neuronal populations, non-invasively in intact brain or reduced preparations. Sensor analysis in healthy and diseased brains might reveal important differences and shed light on the development and progression of nervous system disorders. We review the field of genetically encoded sensors for molecules and cellular events and their potential applicability to the study of nervous system disease.
UR - http://www.scopus.com/inward/record.url?scp=57349122926&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=57349122926&partnerID=8YFLogxK
U2 - 10.1016/j.ddmod.2008.07.003
DO - 10.1016/j.ddmod.2008.07.003
M3 - Article
AN - SCOPUS:57349122926
VL - 5
SP - 27
EP - 35
JO - Drug Discovery Today: Disease Models
JF - Drug Discovery Today: Disease Models
SN - 1740-6757
IS - 1
ER -