Genetic variation in the sand fly salivary protein, SP-15, a potential vaccine candidate against Leishmania major

D. E A Elnalem, C. Meneses, M. Slotman, Gregory C Lanzaro

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

SP-15 is a sandfly salivary protein that provides strong protection against cutaneous leishmaniasis, caused by Leishmania major, and has been proposed as a potential vaccine against this disease. To investigate possible antigenic variation in this protein, we examined genetic polymorphism of SP-15 in 100 Phlebotomus papatasi sandflies, from a natural population from Sudan and four laboratory colonies from Egypt, Jordan, Israel and Saudi Arabia. We found that although many variants of SP-15 may be found in nature, differences among them are minimal (mean ± SD pairwise differences = 1.69 ± 0.83% for forty nucleotide sequences and 3.06 ± 1.13% for thirty amino acid sequence variants). Analysis of proportions of synonymous and non-synonymous substitutions indicated that SP-15 is not under diversifying selection. Our results suggest that a vaccine based on SP-15 protein should result in a uniform immune response.

Original languageEnglish (US)
Pages (from-to)145-150
Number of pages6
JournalInsect Molecular Biology
Volume14
Issue number2
DOIs
StatePublished - Apr 2005

Keywords

  • Leishmaniasis
  • Saliva
  • Sandflies
  • SP-15
  • Vaccines

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

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