Genetic variation in the sand fly salivary protein, SP-15, a potential vaccine candidate against Leishmania major

D. E A Elnalem, C. Meneses, M. Slotman, Gregory C Lanzaro

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

SP-15 is a sandfly salivary protein that provides strong protection against cutaneous leishmaniasis, caused by Leishmania major, and has been proposed as a potential vaccine against this disease. To investigate possible antigenic variation in this protein, we examined genetic polymorphism of SP-15 in 100 Phlebotomus papatasi sandflies, from a natural population from Sudan and four laboratory colonies from Egypt, Jordan, Israel and Saudi Arabia. We found that although many variants of SP-15 may be found in nature, differences among them are minimal (mean ± SD pairwise differences = 1.69 ± 0.83% for forty nucleotide sequences and 3.06 ± 1.13% for thirty amino acid sequence variants). Analysis of proportions of synonymous and non-synonymous substitutions indicated that SP-15 is not under diversifying selection. Our results suggest that a vaccine based on SP-15 protein should result in a uniform immune response.

Original languageEnglish (US)
Pages (from-to)145-150
Number of pages6
JournalInsect Molecular Biology
Volume14
Issue number2
DOIs
StatePublished - Apr 2005

Fingerprint

Salivary Proteins and Peptides
Leishmania major
Psychodidae
Phlebotominae
Sand
Vaccines
Phlebotomus
vaccines
Antigenic Variation
Jordan
genetic variation
Cutaneous Leishmaniasis
Sudan
Saudi Arabia
Egypt
Genetic Polymorphisms
Israel
Polymorphism
Phlebotomus papatasi
cutaneous leishmaniasis

Keywords

  • Leishmaniasis
  • Saliva
  • Sandflies
  • SP-15
  • Vaccines

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Genetic variation in the sand fly salivary protein, SP-15, a potential vaccine candidate against Leishmania major. / Elnalem, D. E A; Meneses, C.; Slotman, M.; Lanzaro, Gregory C.

In: Insect Molecular Biology, Vol. 14, No. 2, 04.2005, p. 145-150.

Research output: Contribution to journalArticle

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