Abstract
The open reading frame 2 (ORF2) of three laboratory strains, the live attenuated vaccine virus, and 18 field isolates of equine arteritis virus (EAV) from Europe and North America was sequenced. The ORF2 of EAV encodes the G(S) protein that is abundantly expressed in infected cells but constitutes less than 2% of the virion protein mass. Variation of ORF2 among the isolates facilitated phylogenetic analysis that largely confirmed results of an earlier study based on sequence divergence of ORF5 of the same isolates of EAV, despite exposure of the proteins encoded by ORF2 (G(S)) and ORE5 (G(L)) to potentially different selective pressures in vivo. The data indicate that the G(S) protein is highly conserved between isolates, considerably more so than the G(L) protein, consistent with an important role of the G(S) protein in virus replication.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 41-52 |
| Number of pages | 12 |
| Journal | Virus Research |
| Volume | 42 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Jun 1996 |
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Keywords
- Equine arteritis virus
- G(S) protein
- Phylogenetic analysis
ASJC Scopus subject areas
- Cancer Research
- Molecular Biology
- Virology
Cite this
Genetic variation in open reading frame 2 of field isolates and laboratory strains of equine arteritis virus. / Hedges, Jodi F.; Balasuriya, Udeni B R; Timoney, Peter J.; McCollum, William H.; Maclachlan, Nigel J.
In: Virus Research, Vol. 42, No. 1-2, 06.1996, p. 41-52.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genetic variation in open reading frame 2 of field isolates and laboratory strains of equine arteritis virus
AU - Hedges, Jodi F.
AU - Balasuriya, Udeni B R
AU - Timoney, Peter J.
AU - McCollum, William H.
AU - Maclachlan, Nigel J
PY - 1996/6
Y1 - 1996/6
N2 - The open reading frame 2 (ORF2) of three laboratory strains, the live attenuated vaccine virus, and 18 field isolates of equine arteritis virus (EAV) from Europe and North America was sequenced. The ORF2 of EAV encodes the G(S) protein that is abundantly expressed in infected cells but constitutes less than 2% of the virion protein mass. Variation of ORF2 among the isolates facilitated phylogenetic analysis that largely confirmed results of an earlier study based on sequence divergence of ORF5 of the same isolates of EAV, despite exposure of the proteins encoded by ORF2 (G(S)) and ORE5 (G(L)) to potentially different selective pressures in vivo. The data indicate that the G(S) protein is highly conserved between isolates, considerably more so than the G(L) protein, consistent with an important role of the G(S) protein in virus replication.
AB - The open reading frame 2 (ORF2) of three laboratory strains, the live attenuated vaccine virus, and 18 field isolates of equine arteritis virus (EAV) from Europe and North America was sequenced. The ORF2 of EAV encodes the G(S) protein that is abundantly expressed in infected cells but constitutes less than 2% of the virion protein mass. Variation of ORF2 among the isolates facilitated phylogenetic analysis that largely confirmed results of an earlier study based on sequence divergence of ORF5 of the same isolates of EAV, despite exposure of the proteins encoded by ORF2 (G(S)) and ORE5 (G(L)) to potentially different selective pressures in vivo. The data indicate that the G(S) protein is highly conserved between isolates, considerably more so than the G(L) protein, consistent with an important role of the G(S) protein in virus replication.
KW - Equine arteritis virus
KW - G(S) protein
KW - Phylogenetic analysis
UR - http://www.scopus.com/inward/record.url?scp=0030014227&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030014227&partnerID=8YFLogxK
U2 - 10.1016/0168-1702(96)01294-4
DO - 10.1016/0168-1702(96)01294-4
M3 - Article
VL - 42
SP - 41
EP - 52
JO - Virus Research
JF - Virus Research
SN - 0168-1702
IS - 1-2
ER -