Abstract
The epizootic hemorrhagic disease (EHD) viruses, which are endemic in the southeastern United States, are the causative agents of an important clinical disease in wild and captive ruminants. In order to elucidate the molecular epidemiology of EHD serotype 2, the sequences of portions of the S10 and L2 gene segments (which encode the NS3 viral egress protein and the VP2 surface protein, respectively) of viral isolates made from white-tailed deer over 23 years(1978-2001) were determined and compared using phylogenetic analyses. Both loci demonstrated very little genetic variation among isolates, with a 94-100% nucleotide identity among isolates at the S10 locus (98-100% amino acid conservation), and an 89.1-100% identity among isolates at the L2 locus (87.6-100% amino acid conservation). The grouping of isolates within the resulting cladograms appeared nearly random with respect to time of isolation and geographic origin. One exception to this was a distinctive grouping of some isolates from 1996 to 1997, which formed a separate subclade in both the S10 and L2 cladograms.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 157-165 |
| Number of pages | 9 |
| Journal | Infection, Genetics and Evolution |
| Volume | 5 |
| Issue number | 2 |
| DOIs | |
| State | Published - Mar 2005 |
Fingerprint
Keywords
- Arbovirus
- Culicoides
- Epizootic hemorrhagic disease virus
- Odocoileus virginianus
- Orbivirus
- RNA virus
- Virus evolution
- White-tailed deer
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Genetics
- Microbiology
- Infectious Diseases
Cite this
Genetic variation among epizootic hemorrhagic disease viruses in the southeastern United States : 1978-2001. / Murphy, Molly D.; Howerth, Elizabeth W.; Maclachlan, Nigel J; Stallknecht, David E.
In: Infection, Genetics and Evolution, Vol. 5, No. 2, 03.2005, p. 157-165.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genetic variation among epizootic hemorrhagic disease viruses in the southeastern United States
T2 - 1978-2001
AU - Murphy, Molly D.
AU - Howerth, Elizabeth W.
AU - Maclachlan, Nigel J
AU - Stallknecht, David E.
PY - 2005/3
Y1 - 2005/3
N2 - The epizootic hemorrhagic disease (EHD) viruses, which are endemic in the southeastern United States, are the causative agents of an important clinical disease in wild and captive ruminants. In order to elucidate the molecular epidemiology of EHD serotype 2, the sequences of portions of the S10 and L2 gene segments (which encode the NS3 viral egress protein and the VP2 surface protein, respectively) of viral isolates made from white-tailed deer over 23 years(1978-2001) were determined and compared using phylogenetic analyses. Both loci demonstrated very little genetic variation among isolates, with a 94-100% nucleotide identity among isolates at the S10 locus (98-100% amino acid conservation), and an 89.1-100% identity among isolates at the L2 locus (87.6-100% amino acid conservation). The grouping of isolates within the resulting cladograms appeared nearly random with respect to time of isolation and geographic origin. One exception to this was a distinctive grouping of some isolates from 1996 to 1997, which formed a separate subclade in both the S10 and L2 cladograms.
AB - The epizootic hemorrhagic disease (EHD) viruses, which are endemic in the southeastern United States, are the causative agents of an important clinical disease in wild and captive ruminants. In order to elucidate the molecular epidemiology of EHD serotype 2, the sequences of portions of the S10 and L2 gene segments (which encode the NS3 viral egress protein and the VP2 surface protein, respectively) of viral isolates made from white-tailed deer over 23 years(1978-2001) were determined and compared using phylogenetic analyses. Both loci demonstrated very little genetic variation among isolates, with a 94-100% nucleotide identity among isolates at the S10 locus (98-100% amino acid conservation), and an 89.1-100% identity among isolates at the L2 locus (87.6-100% amino acid conservation). The grouping of isolates within the resulting cladograms appeared nearly random with respect to time of isolation and geographic origin. One exception to this was a distinctive grouping of some isolates from 1996 to 1997, which formed a separate subclade in both the S10 and L2 cladograms.
KW - Arbovirus
KW - Culicoides
KW - Epizootic hemorrhagic disease virus
KW - Odocoileus virginianus
KW - Orbivirus
KW - RNA virus
KW - Virus evolution
KW - White-tailed deer
UR - http://www.scopus.com/inward/record.url?scp=11844255791&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=11844255791&partnerID=8YFLogxK
U2 - 10.1016/j.meegid.2004.09.001
DO - 10.1016/j.meegid.2004.09.001
M3 - Article
C2 - 15639748
AN - SCOPUS:11844255791
VL - 5
SP - 157
EP - 165
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
SN - 1567-1348
IS - 2
ER -