Genetic studies of low-abundance human plasma proteins. XIII. Population genetics of C1R complement subcomponent and description of new variants

M. I. Kamboh, Leslie A Lyons, R. E. Ferrell

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Isoelectric focusing and immunoblotting reveals considerable biochemical and genetic variation in the C1R subcomponent of the first complement component. The nature of the intraindividual biochemical variation can be explained by differences in sialic acid content because after digestion with neuraminidase the terminal sialic acids are removed to yield a single major band corresponding to the C1R polypeptide. Plasma samples from a large number of different ethnic groups, consisting of U.S. whites, U.S. blacks, Nigerian blacks, and Inuit, Aleut, and Amerindian populations from the Western Hemisphere have revealed genetically determined charge variation with heterozygous phenotypes consisting of two major asialo bands, indicating that the underlying variation is not due to variation in sialic acid content. Two previously reported common alleles, C1R*1 and C1R*2, have been observed in all studied populations, the notable exception being the Dogrib Indian population, which is devoid of the C1R*2 allele. Several new alleles - designated C1R*3, C1R*4, C1R*5, C1R*6, and C1R*7 - have been observed, with variable frequencies ranging from the occurrence in a single individual and related family members to the polymorphic occurrence of certain alleles in several populations. Of these new alleles, the C1R*5 is of considerable interest in population and anthropological genetics studies. The C1R*5 allele is widely distributed, at a frequency of .03 to .17, in all of the North American aboriginal populations screened. This allele is not present in U.S. whites but is present at a polymorphic frequency in U.S. and Nigerian blacks. The occurrence of the electrophoretically identical C1R*5 allele at polymorphic frequency in North American aboriginals and sporadically in populations of African origin raises interesting questions regarding the origin and possible heterogeneity of this allele.

Original languageEnglish (US)
Pages (from-to)146-153
Number of pages8
JournalAmerican Journal of Human Genetics
Volume44
Issue number1
StatePublished - 1989
Externally publishedYes

Fingerprint

Population Genetics
Blood Proteins
Alleles
Population
N-Acetylneuraminic Acid
Inuits
Sialic Acids
Anthropology
Neuraminidase
Isoelectric Focusing
Ethnic Groups
Immunoblotting
Molecular Biology
Digestion
Phenotype
Peptides

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

@article{ab56778cfe344aa2bbad4b0268f79947,
title = "Genetic studies of low-abundance human plasma proteins. XIII. Population genetics of C1R complement subcomponent and description of new variants",
abstract = "Isoelectric focusing and immunoblotting reveals considerable biochemical and genetic variation in the C1R subcomponent of the first complement component. The nature of the intraindividual biochemical variation can be explained by differences in sialic acid content because after digestion with neuraminidase the terminal sialic acids are removed to yield a single major band corresponding to the C1R polypeptide. Plasma samples from a large number of different ethnic groups, consisting of U.S. whites, U.S. blacks, Nigerian blacks, and Inuit, Aleut, and Amerindian populations from the Western Hemisphere have revealed genetically determined charge variation with heterozygous phenotypes consisting of two major asialo bands, indicating that the underlying variation is not due to variation in sialic acid content. Two previously reported common alleles, C1R*1 and C1R*2, have been observed in all studied populations, the notable exception being the Dogrib Indian population, which is devoid of the C1R*2 allele. Several new alleles - designated C1R*3, C1R*4, C1R*5, C1R*6, and C1R*7 - have been observed, with variable frequencies ranging from the occurrence in a single individual and related family members to the polymorphic occurrence of certain alleles in several populations. Of these new alleles, the C1R*5 is of considerable interest in population and anthropological genetics studies. The C1R*5 allele is widely distributed, at a frequency of .03 to .17, in all of the North American aboriginal populations screened. This allele is not present in U.S. whites but is present at a polymorphic frequency in U.S. and Nigerian blacks. The occurrence of the electrophoretically identical C1R*5 allele at polymorphic frequency in North American aboriginals and sporadically in populations of African origin raises interesting questions regarding the origin and possible heterogeneity of this allele.",
author = "Kamboh, {M. I.} and Lyons, {Leslie A} and Ferrell, {R. E.}",
year = "1989",
language = "English (US)",
volume = "44",
pages = "146--153",
journal = "American Journal of Human Genetics",
issn = "0002-9297",
publisher = "Cell Press",
number = "1",

}

TY - JOUR

T1 - Genetic studies of low-abundance human plasma proteins. XIII. Population genetics of C1R complement subcomponent and description of new variants

AU - Kamboh, M. I.

AU - Lyons, Leslie A

AU - Ferrell, R. E.

PY - 1989

Y1 - 1989

N2 - Isoelectric focusing and immunoblotting reveals considerable biochemical and genetic variation in the C1R subcomponent of the first complement component. The nature of the intraindividual biochemical variation can be explained by differences in sialic acid content because after digestion with neuraminidase the terminal sialic acids are removed to yield a single major band corresponding to the C1R polypeptide. Plasma samples from a large number of different ethnic groups, consisting of U.S. whites, U.S. blacks, Nigerian blacks, and Inuit, Aleut, and Amerindian populations from the Western Hemisphere have revealed genetically determined charge variation with heterozygous phenotypes consisting of two major asialo bands, indicating that the underlying variation is not due to variation in sialic acid content. Two previously reported common alleles, C1R*1 and C1R*2, have been observed in all studied populations, the notable exception being the Dogrib Indian population, which is devoid of the C1R*2 allele. Several new alleles - designated C1R*3, C1R*4, C1R*5, C1R*6, and C1R*7 - have been observed, with variable frequencies ranging from the occurrence in a single individual and related family members to the polymorphic occurrence of certain alleles in several populations. Of these new alleles, the C1R*5 is of considerable interest in population and anthropological genetics studies. The C1R*5 allele is widely distributed, at a frequency of .03 to .17, in all of the North American aboriginal populations screened. This allele is not present in U.S. whites but is present at a polymorphic frequency in U.S. and Nigerian blacks. The occurrence of the electrophoretically identical C1R*5 allele at polymorphic frequency in North American aboriginals and sporadically in populations of African origin raises interesting questions regarding the origin and possible heterogeneity of this allele.

AB - Isoelectric focusing and immunoblotting reveals considerable biochemical and genetic variation in the C1R subcomponent of the first complement component. The nature of the intraindividual biochemical variation can be explained by differences in sialic acid content because after digestion with neuraminidase the terminal sialic acids are removed to yield a single major band corresponding to the C1R polypeptide. Plasma samples from a large number of different ethnic groups, consisting of U.S. whites, U.S. blacks, Nigerian blacks, and Inuit, Aleut, and Amerindian populations from the Western Hemisphere have revealed genetically determined charge variation with heterozygous phenotypes consisting of two major asialo bands, indicating that the underlying variation is not due to variation in sialic acid content. Two previously reported common alleles, C1R*1 and C1R*2, have been observed in all studied populations, the notable exception being the Dogrib Indian population, which is devoid of the C1R*2 allele. Several new alleles - designated C1R*3, C1R*4, C1R*5, C1R*6, and C1R*7 - have been observed, with variable frequencies ranging from the occurrence in a single individual and related family members to the polymorphic occurrence of certain alleles in several populations. Of these new alleles, the C1R*5 is of considerable interest in population and anthropological genetics studies. The C1R*5 allele is widely distributed, at a frequency of .03 to .17, in all of the North American aboriginal populations screened. This allele is not present in U.S. whites but is present at a polymorphic frequency in U.S. and Nigerian blacks. The occurrence of the electrophoretically identical C1R*5 allele at polymorphic frequency in North American aboriginals and sporadically in populations of African origin raises interesting questions regarding the origin and possible heterogeneity of this allele.

UR - http://www.scopus.com/inward/record.url?scp=0024484666&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024484666&partnerID=8YFLogxK

M3 - Article

C2 - 2535773

AN - SCOPUS:0024484666

VL - 44

SP - 146

EP - 153

JO - American Journal of Human Genetics

JF - American Journal of Human Genetics

SN - 0002-9297

IS - 1

ER -