Genetic reassortment of bluetongue virus Serotype 11 strains in the bovine

R. D. Oberst, Jeffrey L Stott, Myra Blanchard, Bennie Osburn

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Reassortants of bluetongue virus Serotype 11 (BTV-11) were isolated from a yearling heifer experimentally infected with two electrophoretically different strains (UC-2 and UC-8) by subcutaneous inoculation. Viruses were recovered by direct titration of sonicated blood samples onto Vero cell monolayers, which were overlaid with agarose and later plaque purified. The parental electropherotype of UC-8 was identified as the predominant virus strain during the infection; UC-2 was not isolated. UC-2 inffectivity was shown by reassortants which contained genome segments that were identical in migration pattern to the parental UC-2 electropherotype. The observations demonstrate that segmental reassortment can occur during mixed infections in the bovine, between strains of the same BTV serotype.

Original languageEnglish (US)
Pages (from-to)11-18
Number of pages8
JournalVeterinary Microbiology
Volume15
Issue number1-2
DOIs
StatePublished - 1987

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

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