Abstract
A number of genetic methods for the isolation, characterization and manipulation of large chromosomal inversions in Salmonella typhimurium are described. One inversion-carrying mutant is characterized in detail and used to demonstrate a number of unique genetic properties of bacterial inversions. -Contrary to expectation, it was found that large inversion mutations can be repaired by generalized transduction. The repair results from the simultaneous introduction of two wild-type transduced fragments into a single recipient cell. Homologous recombination between the two transduced fragments and the two inversion breakpoints causes the inverted segment to be reinverted. This results in regeneration of the wild-type orientation of this chromosome segment. Similar recombination events allow a large inversion mutation to be introduced into a wild-type strain: two transduced fragments from an inversion strain cause recombination events resulting in inversion of a large chromosome segment.-Genetic methods for mapping the extent of a large inversion mutation by generalized transduction are described and tested. The methods are operationally simple and allow good resolution of the two inversion breakpoints.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 517-537 |
| Number of pages | 21 |
| Journal | Genetics |
| Volume | 105 |
| Issue number | 3 |
| State | Published - 1983 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
- Genetics(clinical)
Cite this
Genetic methods for analysis and manipulation of inversion mutations in bacteria. / Schmid, M. B.; Roth, J. R.
In: Genetics, Vol. 105, No. 3, 1983, p. 517-537.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genetic methods for analysis and manipulation of inversion mutations in bacteria
AU - Schmid, M. B.
AU - Roth, J. R.
PY - 1983
Y1 - 1983
N2 - A number of genetic methods for the isolation, characterization and manipulation of large chromosomal inversions in Salmonella typhimurium are described. One inversion-carrying mutant is characterized in detail and used to demonstrate a number of unique genetic properties of bacterial inversions. -Contrary to expectation, it was found that large inversion mutations can be repaired by generalized transduction. The repair results from the simultaneous introduction of two wild-type transduced fragments into a single recipient cell. Homologous recombination between the two transduced fragments and the two inversion breakpoints causes the inverted segment to be reinverted. This results in regeneration of the wild-type orientation of this chromosome segment. Similar recombination events allow a large inversion mutation to be introduced into a wild-type strain: two transduced fragments from an inversion strain cause recombination events resulting in inversion of a large chromosome segment.-Genetic methods for mapping the extent of a large inversion mutation by generalized transduction are described and tested. The methods are operationally simple and allow good resolution of the two inversion breakpoints.
AB - A number of genetic methods for the isolation, characterization and manipulation of large chromosomal inversions in Salmonella typhimurium are described. One inversion-carrying mutant is characterized in detail and used to demonstrate a number of unique genetic properties of bacterial inversions. -Contrary to expectation, it was found that large inversion mutations can be repaired by generalized transduction. The repair results from the simultaneous introduction of two wild-type transduced fragments into a single recipient cell. Homologous recombination between the two transduced fragments and the two inversion breakpoints causes the inverted segment to be reinverted. This results in regeneration of the wild-type orientation of this chromosome segment. Similar recombination events allow a large inversion mutation to be introduced into a wild-type strain: two transduced fragments from an inversion strain cause recombination events resulting in inversion of a large chromosome segment.-Genetic methods for mapping the extent of a large inversion mutation by generalized transduction are described and tested. The methods are operationally simple and allow good resolution of the two inversion breakpoints.
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UR - http://www.scopus.com/inward/citedby.url?scp=0021056590&partnerID=8YFLogxK
M3 - Article
C2 - 6357943
AN - SCOPUS:0021056590
VL - 105
SP - 517
EP - 537
JO - Genetics
JF - Genetics
SN - 0016-6731
IS - 3
ER -