Abstract
A number of genetic methods for the isolation, characterization and manipulation of large chromosomal inversions in Salmonella typhimurium are described. One inversion-carrying mutant is characterized in detail and used to demonstrate a number of unique genetic properties of bacterial inversions. -Contrary to expectation, it was found that large inversion mutations can be repaired by generalized transduction. The repair results from the simultaneous introduction of two wild-type transduced fragments into a single recipient cell. Homologous recombination between the two transduced fragments and the two inversion breakpoints causes the inverted segment to be reinverted. This results in regeneration of the wild-type orientation of this chromosome segment. Similar recombination events allow a large inversion mutation to be introduced into a wild-type strain: two transduced fragments from an inversion strain cause recombination events resulting in inversion of a large chromosome segment.-Genetic methods for mapping the extent of a large inversion mutation by generalized transduction are described and tested. The methods are operationally simple and allow good resolution of the two inversion breakpoints.
Original language | English (US) |
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Pages (from-to) | 517-537 |
Number of pages | 21 |
Journal | Genetics |
Volume | 105 |
Issue number | 3 |
State | Published - 1983 |
Externally published | Yes |
ASJC Scopus subject areas
- Genetics
- Genetics(clinical)