Genetic methods for analysis and manipulation of inversion mutations in bacteria

M. B. Schmid, J. R. Roth

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A number of genetic methods for the isolation, characterization and manipulation of large chromosomal inversions in Salmonella typhimurium are described. One inversion-carrying mutant is characterized in detail and used to demonstrate a number of unique genetic properties of bacterial inversions. -Contrary to expectation, it was found that large inversion mutations can be repaired by generalized transduction. The repair results from the simultaneous introduction of two wild-type transduced fragments into a single recipient cell. Homologous recombination between the two transduced fragments and the two inversion breakpoints causes the inverted segment to be reinverted. This results in regeneration of the wild-type orientation of this chromosome segment. Similar recombination events allow a large inversion mutation to be introduced into a wild-type strain: two transduced fragments from an inversion strain cause recombination events resulting in inversion of a large chromosome segment.-Genetic methods for mapping the extent of a large inversion mutation by generalized transduction are described and tested. The methods are operationally simple and allow good resolution of the two inversion breakpoints.

Original languageEnglish (US)
Pages (from-to)517-537
Number of pages21
JournalGenetics
Volume105
Issue number3
StatePublished - 1983
Externally publishedYes

Fingerprint

Bacteria
Mutation
Genetic Recombination
Chromosomes
Homologous Recombination
Salmonella typhimurium
Regeneration

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Genetic methods for analysis and manipulation of inversion mutations in bacteria. / Schmid, M. B.; Roth, J. R.

In: Genetics, Vol. 105, No. 3, 1983, p. 517-537.

Research output: Contribution to journalArticle

Schmid, MB & Roth, JR 1983, 'Genetic methods for analysis and manipulation of inversion mutations in bacteria', Genetics, vol. 105, no. 3, pp. 517-537.
Schmid, M. B. ; Roth, J. R. / Genetic methods for analysis and manipulation of inversion mutations in bacteria. In: Genetics. 1983 ; Vol. 105, No. 3. pp. 517-537.
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