Genetic mapping of turnip mosaic virus resistance in Lactuca sativa

M. A. Robbins, H. Witsenboer, Richard W Michelmore, J. F. Laliberte, M. G. Fortin

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Presence of the dominant Tu gene in Lactuca sativa is sufficient to confer resistance to infection by turnip mosaic virus (TuMV). In order to obtain an immunological assay for the presence of TuMV in inoculated plants, the TuMV coat protein (CP) gene was cloned by amplification of a cDNA corresponding to the viral genome using degenerate primers designed from conserved potyvirus CP sequences. The TuMV CP was overexpressed in Escherichia coli, and polyclonal antibodies were produced. To locate Tu on the L. sativa genetic map, F3 families from a cross between cvs "Cobbham Green" (resistant to TuMV) and "Calmar" (susceptible) were genotyped for Tu. Families known to be recombinant in the region containing Tu were infected with TuMV and tested by the indirect enzyme-linked immunosorbent assay (ELISA) using the anti-CP serum. This assay placed Tu between two random amplified polymorphic DNA (RAPD) markers and 3.2 cM from Dm5/8 (which confers resistance to Bremia lactucae). Also, bulked segregant analysis was used to screen for additional RAPD markers tightly linked to the Tu locus. Five new markers linked to Tu were identified in this region, and their location on the genetic map was determined using informative recombinants in the region. Six markers were identified as being linked within 2.5 cM of Tu.

Original languageEnglish (US)
Pages (from-to)583-589
Number of pages7
JournalTheoretical And Applied Genetics
Volume89
Issue number5
DOIs
StatePublished - Nov 1994

Keywords

  • Coat protein
  • Disease resistance
  • Molecular markers

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics
  • Plant Science
  • Horticulture
  • Agronomy and Crop Science

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    Robbins, M. A., Witsenboer, H., Michelmore, R. W., Laliberte, J. F., & Fortin, M. G. (1994). Genetic mapping of turnip mosaic virus resistance in Lactuca sativa. Theoretical And Applied Genetics, 89(5), 583-589. https://doi.org/10.1007/BF00222452