Genetic interactions between CDC31 and KAR1, two genes required for duplication of the microtubule organizing center in Saccharomyces cerevisiae

E. A. Vallen, W. Ho, Mark Winey, M. D. Rose

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Abstract

KAR1 encodes an essential component of the yeast spindle pole body (SPB) that is required for karyogamy and SPB duplication. A temperature-sensitive mutation, kar1-Δ17, mapped to a region required for SPB duplication and for localization to the SPB. To identify interacting SPB proteins, we isolated 13 dominant mutations and 3 high copy number plasmids that suppressed the temperature sensitivity of kar1-Δ17. Eleven extragenic suppressor mutations mapped to two linkage groups, DSK1 and DSK2. The extragenic suppressors were specific for SPB duplication and did not suppress karyogamy-defective alleles. The major class, DSK1, consisted of mutations in CDC31. CDC31 is required for SPB duplication and encodes a calmodulin-like protein that is most closely related to caltractin/centrin, a protein associated with the Chlamydomonas basal body. The high copy number suppressor plasmids contained the wild-type CDC31 gene. One CDC31 suppressor allele conferred a temperature-sensitive defect in SPB duplication, which was counter-suppressed by recessive mutations in KAR1. In spite of the evidence for a direct interaction, the strongest CDC31 alleles, as well as both DSK2 alleles, suppressed a complete deletion of KAR1. However, the CDC31 alleles also made the cell supersensitive to KAR1 gene dosage, arguing against a simple bypass mechanism of suppression. We propose a model in which Kar1p helps localize Cdc31p to the SPB and that Cdc31p then initiates SPB duplication via interaction with a downstream effector.

Original languageEnglish (US)
Pages (from-to)407-422
Number of pages16
JournalGenetics
Volume137
Issue number2
StatePublished - Jan 1 1994
Externally publishedYes

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Spindle Pole Bodies
Microtubule-Organizing Center
Gene Duplication
Saccharomyces cerevisiae
Alleles
Mutation
Temperature
Plasmids
Genetic Suppression
Basal Bodies
Chlamydomonas
Proteins
Gene Dosage
Sulfamethoxazole Drug Combination Trimethoprim
Calmodulin
Yeasts

ASJC Scopus subject areas

  • Genetics

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Genetic interactions between CDC31 and KAR1, two genes required for duplication of the microtubule organizing center in Saccharomyces cerevisiae. / Vallen, E. A.; Ho, W.; Winey, Mark; Rose, M. D.

In: Genetics, Vol. 137, No. 2, 01.01.1994, p. 407-422.

Research output: Contribution to journalArticle

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