Genetic individualization of domestic cats using feline STR loci for forensic applications

Marilyn Menotti-Raymond, Victor A. David, J. Claiborne Stephens, Leslie A Lyons, Stephen J. O'Brien

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

A group of ten short tandem repeat (STR) loci suitable for PCR typing from DNA of domestic cats is evaluated for genetic individualization using blinded samples of eight putative feline blood specimens. The ten loci were also typed in a 70 member cat pedigree to demonstrate Mendelian inheritance and independent assortment. A 'match window' or measurement precision estimate was empirically established by determining the maximum gel migration difference among alleles identical by descent in different individuals of the pedigree. Hardy-Weinberg equilibrium and abundant heterozygosity was observed for each locus in cat population samples from Canada and the USA. The probabilities of two unrelated individuals matching by chance (P(m)) at all ten loci was estimated as 1.35 x 10-10. We present a conservative approach to compute, for forensic consideration, the mathematical likelihood of a chance genotypic match between DNA evidence from a crime scene and the suspect composite STiR genotypes for species or populations when genotype frequency information is not available.

Original languageEnglish (US)
Pages (from-to)1039-1051
Number of pages13
JournalJournal of Forensic Sciences
Volume42
Issue number6
StatePublished - Nov 1997
Externally publishedYes

Keywords

  • Feline short tandem repeat forensic panel
  • Forensic science
  • Genetic individualization of domestic cats

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Law

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