Abstract
This study investigated the genetic diversity among plum curculio, Conotrachelus nenuphar (Herbst), populations from 11 different geographic locations by using partial sequences of mitochondrial cytochrome oxidase I (mtCOI) gene. Polymerase chain reaction (PCR) products were 863 bp. In total, 23 unique haplotypes were identified from 50 tested samples. Haplotypes G (n = 5), L (n = 12), and T (n = 13) made up 60% of the 50 samples. Two distinct major clades were found in the phylogenetic analyses. Northern and southern clades were strongly supported (100/83 (MP/ML) and 100/62, respectively) by bootstraps. The mid-southern subclade within the southern clade was also moderately supported by bootstraps (73/57, MP/ML). The results of analyses of molecular variance (AMOVA) are consistent with phylogenetic analysis. The results suggested that the northern and the southern clades correspond with northern strain and southern strain of plum curculio. The three-group model of AMOVA test may suggest that there is a transitional group existing between northern and southern groups. In this study, the mtCOI sequence was highly informative as a molecular marker in that it could distinguish C. nenuphar from northern and southern geographic locations in the eastern United States.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 824-832 |
| Number of pages | 9 |
| Journal | Annals of the Entomological Society of America |
| Volume | 101 |
| Issue number | 5 |
| DOIs | |
| State | Published - Sep 2008 |
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Keywords
- Conotrachelus nenuphar
- MtCOI
- Phylogeography
- Polymorphism
- Strains
ASJC Scopus subject areas
- Insect Science
Cite this
Genetic diversity of plum curculio (Coleoptera : Curculionidae) among geographical populations in the eastern United States. / Zhang, Xing; Tu, Zhijian; Luckhart, Shirley; Pfeiffer, Douglas G.
In: Annals of the Entomological Society of America, Vol. 101, No. 5, 09.2008, p. 824-832.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genetic diversity of plum curculio (Coleoptera
T2 - Curculionidae) among geographical populations in the eastern United States
AU - Zhang, Xing
AU - Tu, Zhijian
AU - Luckhart, Shirley
AU - Pfeiffer, Douglas G.
PY - 2008/9
Y1 - 2008/9
N2 - This study investigated the genetic diversity among plum curculio, Conotrachelus nenuphar (Herbst), populations from 11 different geographic locations by using partial sequences of mitochondrial cytochrome oxidase I (mtCOI) gene. Polymerase chain reaction (PCR) products were 863 bp. In total, 23 unique haplotypes were identified from 50 tested samples. Haplotypes G (n = 5), L (n = 12), and T (n = 13) made up 60% of the 50 samples. Two distinct major clades were found in the phylogenetic analyses. Northern and southern clades were strongly supported (100/83 (MP/ML) and 100/62, respectively) by bootstraps. The mid-southern subclade within the southern clade was also moderately supported by bootstraps (73/57, MP/ML). The results of analyses of molecular variance (AMOVA) are consistent with phylogenetic analysis. The results suggested that the northern and the southern clades correspond with northern strain and southern strain of plum curculio. The three-group model of AMOVA test may suggest that there is a transitional group existing between northern and southern groups. In this study, the mtCOI sequence was highly informative as a molecular marker in that it could distinguish C. nenuphar from northern and southern geographic locations in the eastern United States.
AB - This study investigated the genetic diversity among plum curculio, Conotrachelus nenuphar (Herbst), populations from 11 different geographic locations by using partial sequences of mitochondrial cytochrome oxidase I (mtCOI) gene. Polymerase chain reaction (PCR) products were 863 bp. In total, 23 unique haplotypes were identified from 50 tested samples. Haplotypes G (n = 5), L (n = 12), and T (n = 13) made up 60% of the 50 samples. Two distinct major clades were found in the phylogenetic analyses. Northern and southern clades were strongly supported (100/83 (MP/ML) and 100/62, respectively) by bootstraps. The mid-southern subclade within the southern clade was also moderately supported by bootstraps (73/57, MP/ML). The results of analyses of molecular variance (AMOVA) are consistent with phylogenetic analysis. The results suggested that the northern and the southern clades correspond with northern strain and southern strain of plum curculio. The three-group model of AMOVA test may suggest that there is a transitional group existing between northern and southern groups. In this study, the mtCOI sequence was highly informative as a molecular marker in that it could distinguish C. nenuphar from northern and southern geographic locations in the eastern United States.
KW - Conotrachelus nenuphar
KW - MtCOI
KW - Phylogeography
KW - Polymorphism
KW - Strains
UR - http://www.scopus.com/inward/record.url?scp=54149112553&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=54149112553&partnerID=8YFLogxK
U2 - 10.1603/0013-8746(2008)101[824:GDOPCC]2.0.CO;2
DO - 10.1603/0013-8746(2008)101[824:GDOPCC]2.0.CO;2
M3 - Article
AN - SCOPUS:54149112553
VL - 101
SP - 824
EP - 832
JO - Annals of the Entomological Society of America
JF - Annals of the Entomological Society of America
SN - 0013-8746
IS - 5
ER -