Genetic diversity of coronaviruses in bats in Lao PDR and Cambodia

Audrey Lacroix, Veasna Duong, Vibol Hul, Sorn San, Hull Davun, Keo Omaliss, Sokha Chea, Alexandre Hassanin, Watthana Theppangna, Soubanh Silithammavong, Kongsy Khammavong, Sinpakone Singhalath, Zoe Greatorex, Amanda E. Fine, Tracey Goldstein, Sarah Olson, Damien O. Joly, Lucy Keatts, Philippe Dussart, Aneta AfeltRoger Frutos, Philippe Buchy

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

South-East Asia is a hot spot for emerging zoonotic diseases, and bats have been recognized as hosts for a large number of zoonotic viruses such as Severe Acute Respiratory Syndrome (SARS), responsible for acute respiratory syndrome outbreaks. Thus, it is important to expand our knowledge of the presence of viruses in bats which could represent a risk to humans. Coronaviruses (CoVs) have been reported in bat species from Thailand, China, Indonesia, Taiwan and the Philippines. However no such work was conducted in Cambodia or Lao PDR. Between 2010 and 2013, 1965 bats were therefore sampled at interfaces with human populations in these two countries. They were tested for the presence of coronavirus by consensus reverse transcription-PCR assay. A total of 93 samples (4.7%) from 17 genera of bats tested positive. Sequence analysis revealed the presence of potentially 37 and 56 coronavirus belonging to alpha-coronavirus (αCoV) and beta-CoV (βCoV), respectively. The βCoVs group is known to include some coronaviruses highly pathogenic to human, such as SARS-CoV and MERS-CoV. All coronavirus sequences generated from frugivorous bats (family Pteropodidae) (n = 55) clustered with other bat βCoVs of lineage D, whereas one coronavirus from Pipistrellus coromandra fell in the lineage C of βCoVs which also includes the MERS-CoV. αCoVs were all detected in various genera of insectivorous bats and clustered with diverse bat αCoV sequences previously published. A closely related strain of PEDV, responsible for severe diarrhea in pigs (PEDV-CoV), was detected in 2 Myotis bats. We highlighted the presence and the high diversity of coronaviruses circulating in bats from Cambodia and Lao PDR. Three new bat genera and species were newly identified as host of coronaviruses, namely Macroglossus sp., Megaerops niphanae and Myotis horsfieldii

Original languageEnglish (US)
Pages (from-to)10-18
Number of pages9
JournalInfection, Genetics and Evolution
Volume48
DOIs
StatePublished - Mar 1 2017

Keywords

  • Bats
  • Cambodia
  • Coronaviruses
  • Genetic diversity
  • Lao PDR

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Genetic diversity of coronaviruses in bats in Lao PDR and Cambodia'. Together they form a unique fingerprint.

  • Cite this

    Lacroix, A., Duong, V., Hul, V., San, S., Davun, H., Omaliss, K., Chea, S., Hassanin, A., Theppangna, W., Silithammavong, S., Khammavong, K., Singhalath, S., Greatorex, Z., Fine, A. E., Goldstein, T., Olson, S., Joly, D. O., Keatts, L., Dussart, P., ... Buchy, P. (2017). Genetic diversity of coronaviruses in bats in Lao PDR and Cambodia. Infection, Genetics and Evolution, 48, 10-18. https://doi.org/10.1016/j.meegid.2016.11.029