Genetic diversity among Borrelia burgdorferi isolates from wood rats and kangaroo rats in California

B. C. Zingg, R. N. Brown, R. S. Lane, Rance B Lefebvre

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Twenty-nine Borrelia burgdorferi isolates, obtained from dusky-footed wood rats (Neotoma fuscipes) and California kangaroo rats (Dipodomys californicus) in California, were analyzed genetically. Chromosomal DNA was examined by restriction endonuclease analysis (REA) and gene probe restriction fragment length polymorphism. Pulsed-field gel electrophoresis was used to analyze the plasmid profiles of the isolates. REA, the method with the greatest discrimination, disclosed 24 distinct restriction patterns among the 29 isolates. These restriction patterns were sorted into four restriction fragment length polymorphism groups on the basis of their gene hybridization patterns. Results of the REA and plasmid profile analysis supported this grouping. The degree of genetic diversity among Californian isolates demonstrated by our findings is greater than that previously reported among other groups of North American isolates and is similar or greater than the diversity reported among European isolates.

Original languageEnglish (US)
Pages (from-to)3109-3114
Number of pages6
JournalJournal of Clinical Microbiology
Volume31
Issue number12
StatePublished - 1993

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Dipodomys
Potoroidae
Borrelia burgdorferi
DNA Restriction Enzymes
Restriction Fragment Length Polymorphisms
Plasmids
Sigmodontinae
Pulsed Field Gel Electrophoresis
Genes
DNA

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Genetic diversity among Borrelia burgdorferi isolates from wood rats and kangaroo rats in California. / Zingg, B. C.; Brown, R. N.; Lane, R. S.; Lefebvre, Rance B.

In: Journal of Clinical Microbiology, Vol. 31, No. 12, 1993, p. 3109-3114.

Research output: Contribution to journalArticle

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