Genetic characterization of the pdu operan: Use of 1,2-propanediol in Salmonella typhimurium

Derek Walter, Michael Ailion, John Roth

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Salmonella typhimurium is able to catabolize 1,2-propanediol for use as the sole carbon and energy source; the first enzyme of this pathway requires the cofactor adenosyl cobalamin (Ado-B12). Surprisingly, Salmonella can use propanediol as the sole carbon source only in the presence of oxygen but can synthesize Ado-B12 only anaerobically. To understand this situation, we have studied the pdu operon, which encodes proteins for propanediol degradation. A set of pdu mutants defective in aerobic degradation of propanediol (with exogenous vitamin B12) defines four distinct complementation groups. Mutations in two of these groups (pduC and pduD) eliminate propanediol dehydratase activity. Based on mutant phenotypes, a third complementation group (pduG) appears to encode a cobalamin adenosyl transferase activity. No function has been assigned to the pduJ complementation group. Propionaldehyde dehydrogenase activity is eliminated by mutations in any of the four identified complementation groups, suggesting that this activity may require a complex of proteins encoded by the operon. None of the mutations analyzed affects either of the first two genes of the operon (pduA and pduB), which were identified by DNA sequence analysis. Available data suggest that the pdu operon includes enough DNA for about 15 genes and that the four genetically identified genes are the only ones required for aerobic use of propanediol.

Original languageEnglish (US)
Pages (from-to)1013-1022
Number of pages10
JournalJournal of Bacteriology
Volume179
Issue number4
StatePublished - 1997
Externally publishedYes

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Propylene Glycols
Propylene Glycol
Salmonella typhimurium
Operon
Vitamin B 12
Mutation
Propanediol Dehydratase
Carbon
Genes
Transferases
DNA Sequence Analysis
Salmonella
Proteolysis
Oxidoreductases
Oxygen
Phenotype
DNA
Enzymes
Proteins

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Genetic characterization of the pdu operan : Use of 1,2-propanediol in Salmonella typhimurium. / Walter, Derek; Ailion, Michael; Roth, John.

In: Journal of Bacteriology, Vol. 179, No. 4, 1997, p. 1013-1022.

Research output: Contribution to journalArticle

Walter, Derek ; Ailion, Michael ; Roth, John. / Genetic characterization of the pdu operan : Use of 1,2-propanediol in Salmonella typhimurium. In: Journal of Bacteriology. 1997 ; Vol. 179, No. 4. pp. 1013-1022.
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