Genetic characterization of equine arteritis virus during persistent infection of stallions

Udeni B R Balasuriya, Jodi F. Hedges, Victoria L. Smalley, Andrea Navarrette, William H. McCollum, Peter J. Timoney, Eric J. Snijder, Nigel J Maclachlan

Research output: Contribution to journalArticle

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Abstract

Equine arteritis virus (EAV) causes a persistent infection of the reproductive tract of carrier stallions. The authors determined the complete genome sequences of viruses (CW96 and CW01) that were present 5 years apart in the semen of a carrier stallion (CW). The CW96 and CW01 viruses respectively had only 85.6% and 85.7% nucleotide identity to the published sequence of EAV (EAV030). The CW96 and CW01 viruses had two 1 nt insertions and a single 1 nt deletion in the leader sequence, and a 3 nt coding insertion in ORF1 a; thus their genomes included 12 708 nt as compared to the 12 704 nt in EAV030. Variation between viruses present in the semen of stallion CW and EAV030 was especially marked in the replicase gene (ORF1a and 1b), and the greatest variation occurred in the portion of ORF1a encoding the nsp2 protein. The ORFs 3 and 5, which respectively encode the GP3 and GP5 envelope proteins, showed greatest variation amongst ORFs encoding structural EAV proteins. Comparative sequence analyses of CW96 and CW01 indicated that ORFs 1a, 1b and 7 were highly conserved during persistent infection, whereas there was substantial variation in ORFs 3 and 5. Although the variation that occurs in ORF5 results in the emergence of novel phenotypic viral variants as determined by neutralization assay, all variants were neutralized by high-titre polyclonal equine antisera, suggesting that immune evasion is unlikely to be responsible for the establishment of persistent EAV infection of carrier stallions. Northern blot analyses of RNA extracted from cell culture propagated viruses isolated from 10 different persistently infected stallions failed to demonstrate any large genomic deletions, suggesting that defective interfering particles are also unlikely to be important in either the maintenance or clearance of persistent EAV infection of the reproductive tract of carrier stallions.

Original languageEnglish (US)
Pages (from-to)379-390
Number of pages12
JournalJournal of General Virology
Volume85
Issue number2
DOIs
StatePublished - Feb 2004

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Equine Arteritis Virus
Open Reading Frames
Viruses
Infection
Virus Diseases
Semen
Genome
Reproductive Tract Infections
Defective Viruses
Immune Evasion
Proteins
Northern Blotting
Horses
Sequence Analysis
Immune Sera
Nucleotides
Cell Culture Techniques
Maintenance
RNA
Genes

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Balasuriya, U. B. R., Hedges, J. F., Smalley, V. L., Navarrette, A., McCollum, W. H., Timoney, P. J., ... Maclachlan, N. J. (2004). Genetic characterization of equine arteritis virus during persistent infection of stallions. Journal of General Virology, 85(2), 379-390. https://doi.org/10.1099/vir.0.19545-0

Genetic characterization of equine arteritis virus during persistent infection of stallions. / Balasuriya, Udeni B R; Hedges, Jodi F.; Smalley, Victoria L.; Navarrette, Andrea; McCollum, William H.; Timoney, Peter J.; Snijder, Eric J.; Maclachlan, Nigel J.

In: Journal of General Virology, Vol. 85, No. 2, 02.2004, p. 379-390.

Research output: Contribution to journalArticle

Balasuriya, UBR, Hedges, JF, Smalley, VL, Navarrette, A, McCollum, WH, Timoney, PJ, Snijder, EJ & Maclachlan, NJ 2004, 'Genetic characterization of equine arteritis virus during persistent infection of stallions', Journal of General Virology, vol. 85, no. 2, pp. 379-390. https://doi.org/10.1099/vir.0.19545-0
Balasuriya UBR, Hedges JF, Smalley VL, Navarrette A, McCollum WH, Timoney PJ et al. Genetic characterization of equine arteritis virus during persistent infection of stallions. Journal of General Virology. 2004 Feb;85(2):379-390. https://doi.org/10.1099/vir.0.19545-0
Balasuriya, Udeni B R ; Hedges, Jodi F. ; Smalley, Victoria L. ; Navarrette, Andrea ; McCollum, William H. ; Timoney, Peter J. ; Snijder, Eric J. ; Maclachlan, Nigel J. / Genetic characterization of equine arteritis virus during persistent infection of stallions. In: Journal of General Virology. 2004 ; Vol. 85, No. 2. pp. 379-390.
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