Genetic and virulence characterization of Flavobacterium columnare from channel catfish (Ictalurus punctatus)

Esteban Soto Martinez, M. J. Mauel, A. Karsi, M. L. Lawrence

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Aim: To develop a method for conducting pulsed-field gel electrophoresis (PFGE) on Flavobacterium columnare, to use PFGE to characterize F. columnare channel catfish isolates, and to determine whether variation in pathogenic potential exists in F. columnare isolates from channel catfish. Methods and Results: On the basis of PFGE-derived profiles, similarity dendrograms constructed for more than 30 F. columnare isolates showed two major genetic groups with more than 60% similarity. Channel catfish fingerlings challenged with PFGE group A isolates by bath immersion had significantly higher average mortalities (>60%) than fish challenged with PFGE group B isolates (<9%). However, abrasion and skin mucus removal made channel catfish fingerlings susceptible to disease caused by group B isolates following immersion exposure. Conclusion: Our results suggest that two genetic divisions of F. columnare channel catfish isolates exist, and that isolates in PFGE group A isolates tend to be more pathogenic to immunocompetent channel catfish fingerlings than PFGE group B isolates. Significance and Impact of the Study: PFGE is a potentially useful tool for determining whether F. columnare isolates are more likely to be primary or secondary pathogens. Pathogenesis research for columnaris disease in catfish should focus on pathogenic isolates from PFGE group A.

Original languageEnglish (US)
Pages (from-to)1302-1310
Number of pages9
JournalJournal of Applied Microbiology
Volume104
Issue number5
DOIs
StatePublished - May 2008
Externally publishedYes

Fingerprint

Flavobacterium columnare
Ictaluridae
Flavobacterium
Pulsed Field Gel Electrophoresis
Ictalurus punctatus
pulsed-field gel electrophoresis
Virulence
virulence
fingerlings
Immersion
Catfishes
biological resistance
Mucus
mucus
Baths
skin (animal)
catfish
Fishes
pathogenicity
pathogenesis

Keywords

  • Aquaculture
  • Channel catfish
  • Flavobacterium columnare
  • Pulsed-field gel electrophoresis
  • Virulence

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Genetic and virulence characterization of Flavobacterium columnare from channel catfish (Ictalurus punctatus). / Soto Martinez, Esteban; Mauel, M. J.; Karsi, A.; Lawrence, M. L.

In: Journal of Applied Microbiology, Vol. 104, No. 5, 05.2008, p. 1302-1310.

Research output: Contribution to journalArticle

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