Genetic and environmental factors affecting the de novo appearance of the [PSI+] prion in Saccharomyces cerevisiae

Irina L. Derkatch, Michael E. Bradley, Ping Zhou, Yury O. Chernoff, Susan W. Liebman

Research output: Contribution to journalArticle

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Abstract

It has previously been shown that yeast prion [PSI+] is cured by GuHCl, although reports on reversibility of curing were contradictory. Here we show that GuHCl treatment of both [PSI+] and [psi-] yeast strains results in two classes of [psi-] derivatives: Pin+, in which [PSI+] can be reinduced by Sup35p overproduction, and Pin-, in which overexpression of the complete SUP35 gene does not lead to the [PSI+] appearance. However, in both Pin+ and Pin- derivatives [PSI+] is reinduced by overproduction of a short Sup35p N-terminal fragment, thus, in principle, [PSI+] curing remains reversible in both cases. Neither suppression nor growth inhibition caused by SUP35 overexpression in Pin+ [psi-] derivatives are observed in Pin- [psi-] derivatives. Genetic analyses show that the Pin+ phenotype is determined by a non-Mendelian factor, which, unlike the [PSI+] prion, is independent of the Sup35p N-terminal domain. A Pin- [psi-] derivative was also generated by transient inactivation of the heat shock protein, Hsp104, while [PSI+] curing by Hsp104 overproduction resulted exclusively in Pin+ [psi-] derivatives. We hypothesize that in addition to the [PSI+] prion- determining domain in the Sup35p N-terminus, there is another self- propagating conformational determinant in the C-proximal part of Sup35p and that this second prion is responsible for the Pin+ phenotype.

Original languageEnglish (US)
Pages (from-to)507-519
Number of pages13
JournalGenetics
Volume147
Issue number2
StatePublished - Oct 1 1997
Externally publishedYes

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Prions
Saccharomyces cerevisiae
Yeasts
Phenotype
Heat-Shock Proteins
Growth
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Derkatch, I. L., Bradley, M. E., Zhou, P., Chernoff, Y. O., & Liebman, S. W. (1997). Genetic and environmental factors affecting the de novo appearance of the [PSI+] prion in Saccharomyces cerevisiae. Genetics, 147(2), 507-519.

Genetic and environmental factors affecting the de novo appearance of the [PSI+] prion in Saccharomyces cerevisiae. / Derkatch, Irina L.; Bradley, Michael E.; Zhou, Ping; Chernoff, Yury O.; Liebman, Susan W.

In: Genetics, Vol. 147, No. 2, 01.10.1997, p. 507-519.

Research output: Contribution to journalArticle

Derkatch, IL, Bradley, ME, Zhou, P, Chernoff, YO & Liebman, SW 1997, 'Genetic and environmental factors affecting the de novo appearance of the [PSI+] prion in Saccharomyces cerevisiae', Genetics, vol. 147, no. 2, pp. 507-519.
Derkatch, Irina L. ; Bradley, Michael E. ; Zhou, Ping ; Chernoff, Yury O. ; Liebman, Susan W. / Genetic and environmental factors affecting the de novo appearance of the [PSI+] prion in Saccharomyces cerevisiae. In: Genetics. 1997 ; Vol. 147, No. 2. pp. 507-519.
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