Abstract
Pathogenic leptospires of serovar hardjo isolated from North American cattle were compared genetically and antigenically to reference strain hardjoprajitno of the Sejroe serogroup. Guanine-plus-cytosine (G + C) contents were determined for the genomes, and microscopic agglutination, Western blotting (immunoblotting), and immunoprecipitation were used to characterize antigenic relatedness. Major differences were demonstrated between the isolates and the reference strain. The G + C content of the reference strain was calculated to be approximately 34 ± 1%, and those of the isolates were calculated at 39 ± 1%. Antigenic differences between the isolates and the reference strain were identified by using rabbit immune serum raised against a hardjo isolate exhaustively adsorbed with hardjoprajitno whole and sonicated cells. Western blot analysis and immunoprecipitation using this adsorbed serum revealed antigens apparently unique for the hardjo isolates. Microscopic agglutination with the adsorbed rabbit serum did not agglutinate hardjoprajitno when diluted 1:2 but agglutinated bovine isolates to a 1:32 dilution. Bovine antiserum raised against the isolates was also used to identify antigens by immunoprecipitation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2094-2097 |
| Number of pages | 4 |
| Journal | Journal of Clinical Microbiology |
| Volume | 25 |
| Issue number | 11 |
| State | Published - 1987 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Microbiology
- Microbiology (medical)
Cite this
Genetic and antigenic differences of serologically indistinguishable leptospires of serovar hardjo. / Lefebvre, Rance B; Thiermann, A. B.; Foley, J.
In: Journal of Clinical Microbiology, Vol. 25, No. 11, 1987, p. 2094-2097.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genetic and antigenic differences of serologically indistinguishable leptospires of serovar hardjo
AU - Lefebvre, Rance B
AU - Thiermann, A. B.
AU - Foley, J.
PY - 1987
Y1 - 1987
N2 - Pathogenic leptospires of serovar hardjo isolated from North American cattle were compared genetically and antigenically to reference strain hardjoprajitno of the Sejroe serogroup. Guanine-plus-cytosine (G + C) contents were determined for the genomes, and microscopic agglutination, Western blotting (immunoblotting), and immunoprecipitation were used to characterize antigenic relatedness. Major differences were demonstrated between the isolates and the reference strain. The G + C content of the reference strain was calculated to be approximately 34 ± 1%, and those of the isolates were calculated at 39 ± 1%. Antigenic differences between the isolates and the reference strain were identified by using rabbit immune serum raised against a hardjo isolate exhaustively adsorbed with hardjoprajitno whole and sonicated cells. Western blot analysis and immunoprecipitation using this adsorbed serum revealed antigens apparently unique for the hardjo isolates. Microscopic agglutination with the adsorbed rabbit serum did not agglutinate hardjoprajitno when diluted 1:2 but agglutinated bovine isolates to a 1:32 dilution. Bovine antiserum raised against the isolates was also used to identify antigens by immunoprecipitation.
AB - Pathogenic leptospires of serovar hardjo isolated from North American cattle were compared genetically and antigenically to reference strain hardjoprajitno of the Sejroe serogroup. Guanine-plus-cytosine (G + C) contents were determined for the genomes, and microscopic agglutination, Western blotting (immunoblotting), and immunoprecipitation were used to characterize antigenic relatedness. Major differences were demonstrated between the isolates and the reference strain. The G + C content of the reference strain was calculated to be approximately 34 ± 1%, and those of the isolates were calculated at 39 ± 1%. Antigenic differences between the isolates and the reference strain were identified by using rabbit immune serum raised against a hardjo isolate exhaustively adsorbed with hardjoprajitno whole and sonicated cells. Western blot analysis and immunoprecipitation using this adsorbed serum revealed antigens apparently unique for the hardjo isolates. Microscopic agglutination with the adsorbed rabbit serum did not agglutinate hardjoprajitno when diluted 1:2 but agglutinated bovine isolates to a 1:32 dilution. Bovine antiserum raised against the isolates was also used to identify antigens by immunoprecipitation.
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M3 - Article
C2 - 2447118
AN - SCOPUS:0023639969
VL - 25
SP - 2094
EP - 2097
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
SN - 0095-1137
IS - 11
ER -