Genetic and antigenic characterization of Borrelia coriaceae, putative agent of epizootic bovine abortion

Rance B Lefebvre, G. C. Perng

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Borrelia coriaceae was characterized genetically and antigenically by utilizing the following techniques: restriction endonuclease analysis, Southern blotting and genomic hybridization, pulsed-field electrophoresis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and immunoblotting. The B. coriaceae genome unique and characteristic banding patterns both by agarose gel electrophoresis and by hybridization when compared with several Borrelia burgdorferi isolates. Pulsed-field gel electrophoresis demonstrated several linear plasmids ranging from 65 to 30 kilobase pairs. Cross-reaction with B. burgdorferi ranging from 21 to 26 kilodaltons were demonstrated by immunoblotting with rabbit anti-B. coriaceae antiserum. However, most B. coriaceae antigens were quite distinct when compared with B. burgdorferi and Leptospira interrogans antigens.

Original languageEnglish (US)
Pages (from-to)389-393
Number of pages5
JournalJournal of Clinical Microbiology
Volume27
Issue number3
StatePublished - 1989

Fingerprint

Borrelia
Borrelia burgdorferi
Immunoblotting
Leptospira interrogans
Nucleic Acid Hybridization
Antigens
Agar Gel Electrophoresis
Pulsed Field Gel Electrophoresis
DNA Restriction Enzymes
Cross Reactions
Southern Blotting
Sodium Dodecyl Sulfate
Electrophoresis
Immune Sera
Polyacrylamide Gel Electrophoresis
Plasmids
Genome
Rabbits

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Genetic and antigenic characterization of Borrelia coriaceae, putative agent of epizootic bovine abortion. / Lefebvre, Rance B; Perng, G. C.

In: Journal of Clinical Microbiology, Vol. 27, No. 3, 1989, p. 389-393.

Research output: Contribution to journalArticle

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