Genetic and antigenic characterization of Borrelia coriaceae, putative agent of epizootic bovine abortion

Rance B Lefebvre, G. C. Perng

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Borrelia coriaceae was characterized genetically and antigenically by utilizing the following techniques: restriction endonuclease analysis, Southern blotting and genomic hybridization, pulsed-field electrophoresis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and immunoblotting. The B. coriaceae genome unique and characteristic banding patterns both by agarose gel electrophoresis and by hybridization when compared with several Borrelia burgdorferi isolates. Pulsed-field gel electrophoresis demonstrated several linear plasmids ranging from 65 to 30 kilobase pairs. Cross-reaction with B. burgdorferi ranging from 21 to 26 kilodaltons were demonstrated by immunoblotting with rabbit anti-B. coriaceae antiserum. However, most B. coriaceae antigens were quite distinct when compared with B. burgdorferi and Leptospira interrogans antigens.

Original languageEnglish (US)
Pages (from-to)389-393
Number of pages5
JournalJournal of Clinical Microbiology
Volume27
Issue number3
StatePublished - 1989

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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