Genetic analysis of autoimmune gld mice. I. Identification of a restriction fragment length polymorphism closely linked to the gld mutation within a conserved linkage group

Michael F Seldin, H. C. Morse, J. P. Reeves, C. L. Scribner, R. C. LeBoeuf, A. D. Steinberg

Research output: Contribution to journalArticle

161 Scopus citations

Abstract

A linkage map of distal mouse chromosome 1 was generated using restriction fragment length polymorphism (RFLP) analysis of DNA prepared from 95 [C3H-gld/gld x Mus spretus)F1 x C3H-gld/gld] backcross mice. The gene order was: (centromere) C4bp, Ren-1,2, Ly-5, [At-3/gld], Apoa-2/Ly-17, Spna-1 (telomere). All mice expressing the phenotype of gld homozygotes were homozygous for the At-3 RFLP characteristic of C3H mice and none of the mice heterozygous for At-3 RFLPs had characteristics of gld homozygotes, demonstrating close linkage between these genes. The identification of an RFLP closely linked to the gld gene provides a starting point for the identification of a genetic defect that results in abnormal T cells and autoimmune disease.

Original languageEnglish (US)
Pages (from-to)688-693
Number of pages6
JournalJournal of Experimental Medicine
Volume167
Issue number2
StatePublished - 1988
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

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