Genetic analysis and antimicrobial susceptibility of Francisella noatunensis subsp. orientalis (syn. F. asiatica) isolates from fish

Esteban Soto Martinez, Matt Griffin, Judy Wiles, John P. Hawke

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Francisella noatunensis subsp. orientalis (syn. F. asiatica) (Fno) is an emergent fish pathogen that causes acute to chronic disease in a wide variety of freshwater, brackish and marine fish. Due to the emergent nature of this bacterium, established protocols to measure antimicrobial susceptibility are lacking. In this project we compare three different methods to examine the antimicrobial susceptibility (Etest, broth microdilution and disk diffusion) of 10 different isolates of Fno from two different fish species and four different geographic outbreaks from 2006 to 2010. PCR mediated genomic fingerprinting (rep-PCR) performed on the different isolates confirmed genetic homogeneity amongst the different isolates. The in vitro susceptibility data presented here provides important baseline data for future research monitoring the development of antibiotic resistance among Fno isolates as well as provides invaluable data for the development of potential therapeutics.

Original languageEnglish (US)
Pages (from-to)407-412
Number of pages6
JournalVeterinary Microbiology
Volume154
Issue number3-4
DOIs
StatePublished - Jan 27 2012
Externally publishedYes

Keywords

  • Antimicrobial
  • Francisella asiatica
  • Francisella noatunensis subsp. orientalis
  • Tilapia
  • Treatment

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Fingerprint Dive into the research topics of 'Genetic analysis and antimicrobial susceptibility of Francisella noatunensis subsp. orientalis (syn. F. asiatica) isolates from fish'. Together they form a unique fingerprint.

  • Cite this