Genetic analyses of Tanzanian local chicken ecotypes challenged with newcastle disease virus

Muhammed Walugembe, James R. Mushi, Esinam N. Amuzu-Aweh, Gaspar H. Chiwanga, Peter L. Msoffe, Ying Wang, Perot Saelao, Terra Kelly, Rodrigo A Gallardo, Huaijun Zhou, Susan J. Lamont, Amandus P. Muhairwa, Jack C.M. Dekkers

Research output: Contribution to journalArticle

Abstract

Newcastle Disease (ND) is a continuing global threat to domestic poultry, especially in developing countries, where severe outbreaks of velogenic ND virus (NDV) often cause major economic losses to households. Local chickens are of great importance to rural family livelihoods through provision of high-quality protein. To investigate the genetic basis of host response to NDV, three popular Tanzanian chicken ecotypes (regional populations) were challenged with a lentogenic (vaccine) strain of NDV at 28 days of age. Various host response phenotypes, including anti-NDV antibody levels (pre-infection and 10 days post-infection, dpi), and viral load (2 and 6 dpi) were measured, in addition to growth rate. We estimated genetic parameters and conducted genome-wide association study analyses by genotyping 1399 chickens using the Affymetrix 600K chicken SNP chip. Estimates of heritability of the evaluated traits were moderate (0.18–0.35). Five quantitative trait loci (QTL) associated with growth and/or response to NDV were identified by single-SNP analyses, with some regions explaining ≥1% of genetic variance based on the Bayes-B method. Immune related genes, such as ETS1, TIRAP, and KIRREL3, were located in regions associated with viral load at 6 dpi. The moderate estimates of heritability and identified QTL indicate that NDV response traits may be improved through selective breeding of chickens to enhance increased NDV resistance and vaccine efficacy in Tanzanian local ecotypes.

Original languageEnglish (US)
Article number546
JournalGenes
Volume10
Issue number7
DOIs
StatePublished - Jul 1 2019

Fingerprint

Ecotype
Newcastle disease virus
Chickens
Viruses
Quantitative Trait Loci
Genome-Wide Association Study
Infection
Viral Load
Single Nucleotide Polymorphism
Vaccines
Newcastle Disease
Poultry
Growth
Developing Countries
Disease Outbreaks
Economics
Phenotype
Antibodies
Population
Genes

Keywords

  • GWAS
  • Immune response
  • NDV
  • QTL
  • Tanzanian local ecotypes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Walugembe, M., Mushi, J. R., Amuzu-Aweh, E. N., Chiwanga, G. H., Msoffe, P. L., Wang, Y., ... Dekkers, J. C. M. (2019). Genetic analyses of Tanzanian local chicken ecotypes challenged with newcastle disease virus. Genes, 10(7), [546]. https://doi.org/10.3390/genes10070546

Genetic analyses of Tanzanian local chicken ecotypes challenged with newcastle disease virus. / Walugembe, Muhammed; Mushi, James R.; Amuzu-Aweh, Esinam N.; Chiwanga, Gaspar H.; Msoffe, Peter L.; Wang, Ying; Saelao, Perot; Kelly, Terra; Gallardo, Rodrigo A; Zhou, Huaijun; Lamont, Susan J.; Muhairwa, Amandus P.; Dekkers, Jack C.M.

In: Genes, Vol. 10, No. 7, 546, 01.07.2019.

Research output: Contribution to journalArticle

Walugembe, M, Mushi, JR, Amuzu-Aweh, EN, Chiwanga, GH, Msoffe, PL, Wang, Y, Saelao, P, Kelly, T, Gallardo, RA, Zhou, H, Lamont, SJ, Muhairwa, AP & Dekkers, JCM 2019, 'Genetic analyses of Tanzanian local chicken ecotypes challenged with newcastle disease virus', Genes, vol. 10, no. 7, 546. https://doi.org/10.3390/genes10070546
Walugembe M, Mushi JR, Amuzu-Aweh EN, Chiwanga GH, Msoffe PL, Wang Y et al. Genetic analyses of Tanzanian local chicken ecotypes challenged with newcastle disease virus. Genes. 2019 Jul 1;10(7). 546. https://doi.org/10.3390/genes10070546
Walugembe, Muhammed ; Mushi, James R. ; Amuzu-Aweh, Esinam N. ; Chiwanga, Gaspar H. ; Msoffe, Peter L. ; Wang, Ying ; Saelao, Perot ; Kelly, Terra ; Gallardo, Rodrigo A ; Zhou, Huaijun ; Lamont, Susan J. ; Muhairwa, Amandus P. ; Dekkers, Jack C.M. / Genetic analyses of Tanzanian local chicken ecotypes challenged with newcastle disease virus. In: Genes. 2019 ; Vol. 10, No. 7.
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