Genes required for mitotic spindle assembly in Drosophila S2 cells

Gohta Goshima, Roy Wollman, Sarah S. Goodwin, Nan Zhang, Jonathan M. Scholey, Ronald D. Vale, Nico Stuurman

Research output: Contribution to journalArticle

385 Scopus citations

Abstract

The formation of a metaphase spindle, a bipolar microtubule array with centrally aligned chromosomes, is a prerequisite for the faithful segregation of a cell's genetic material. Using a full-genome RNA interference screen of Drosophila S2 cells, we identified about 200 genes that contribute to spindle assembly, more than half of which were unexpected. The screen, in combination with a variety of secondary assays, led to new insights into how spindle microtubules are generated; how centrosomes are positioned; and how centrioles, centrosomes, and kinetochores are assembled.

Original languageEnglish (US)
Pages (from-to)417-421
Number of pages5
JournalScience
Volume316
Issue number5823
DOIs
StatePublished - Apr 20 2007

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    Goshima, G., Wollman, R., Goodwin, S. S., Zhang, N., Scholey, J. M., Vale, R. D., & Stuurman, N. (2007). Genes required for mitotic spindle assembly in Drosophila S2 cells. Science, 316(5823), 417-421. https://doi.org/10.1126/science.1141314