Genes required for mitotic spindle assembly in Drosophila S2 cells

Gohta Goshima, Roy Wollman, Sarah S. Goodwin, Nan Zhang, Jonathan M. Scholey, Ronald D. Vale, Nico Stuurman

Research output: Contribution to journalArticlepeer-review

419 Scopus citations


The formation of a metaphase spindle, a bipolar microtubule array with centrally aligned chromosomes, is a prerequisite for the faithful segregation of a cell's genetic material. Using a full-genome RNA interference screen of Drosophila S2 cells, we identified about 200 genes that contribute to spindle assembly, more than half of which were unexpected. The screen, in combination with a variety of secondary assays, led to new insights into how spindle microtubules are generated; how centrosomes are positioned; and how centrioles, centrosomes, and kinetochores are assembled.

Original languageEnglish (US)
Pages (from-to)417-421
Number of pages5
Issue number5823
StatePublished - Apr 20 2007

ASJC Scopus subject areas

  • General


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