Genes regulated in human breast cancer cells overexpressing manganese-containing superoxide dismutase

Zhongkui Li, Alexander Khaletskiy, Jianyi Wang, Jeffrey Y C Wong, Larry W. Oberley, Jian-Jian Li

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The mitochondrial antioxidant enzyme manganese-containing superoxide dismutase (MnSOD) functions as a tumor suppressor gene. Reconstitution of MnSOD expression in several human cancer cell lines leads to reversion of malignancy and induces a resistant phenotype to the cytotoxic effects of TNF and hyperthermia. The signaling pathways that underlie these phenotypic changes in MnSOD-overexpressing cells are unknown, although alterations in the activity of several redox-sensitive transcription factors, including AP-1 and NF-κB, have been observed. To determine the downstream signaling molecules involved in MnSOD-induced cell resistant phenotype, in the present study we analyzed the expression profile of several groups of genes related to stress response, DNA repair, and apoptosis, in a human breast cancer MCF-7 cell line overexpressing MnSOD (MCF+SOD). Of 588 genes examined, 5 (0.85%) were up-regulated (2-42-fold), and 11 (1.9%) were down-regulated (2-33-fold) in the MCF+SOD cells compared to the parental MCF-7 cells. The five up-regulated genes were MET, GADD153, CD9, α-catenin and plakoglobin. The genes with the most significant down-regulation included: vascular endothelial growth factor receptor 1, TNF-α converting enzyme, and interleukin-1β. GADD153 (involved in the repair of DNA double strand breaks) showed a 33-fold increase in microarray analysis and these results were confirmed by RT-PCR. To further determine the specificity in MnSOD-induced gene regulation, MCF+SOD cells were stably transfected with an antisense MnSOD sequence whose expression was controlled by a tetracycline-inducible regulator. Expression of three up-regulated genes was measured after induction of antisense MnSOD expression. Interestingly, expression level of GADD153 but not MET or CD9 was reduced 24 h after antisense MnSOD induction. Together, these results suggest that reconstitution of MnSOD in tumor cells can specifically modulate the expression of down-stream effector genes. GADD153 and other elements observed in the MCF+SOD cells may play a key role in signaling the MnSOD-induced cell phenotypic change.

Original languageEnglish (US)
Pages (from-to)260-267
Number of pages8
JournalFree Radical Biology and Medicine
Volume30
Issue number3
DOIs
StatePublished - Feb 1 2001
Externally publishedYes

Fingerprint

Manganese
Superoxide Dismutase
Genes
Cells
Breast Neoplasms
MCF-7 Cells
Tumors
Repair
gamma Catenin
Vascular Endothelial Growth Factor Receptor-1
Phenotype
Cell Line
Catenins
Caspase 1
Neoplasms
Double-Stranded DNA Breaks
DNA
Transcription Factor AP-1
Microarray Analysis
Microarrays

Keywords

  • DNA microarray
  • Free radicals
  • GADD153
  • Human breast cancer
  • MnSOD

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Genes regulated in human breast cancer cells overexpressing manganese-containing superoxide dismutase. / Li, Zhongkui; Khaletskiy, Alexander; Wang, Jianyi; Wong, Jeffrey Y C; Oberley, Larry W.; Li, Jian-Jian.

In: Free Radical Biology and Medicine, Vol. 30, No. 3, 01.02.2001, p. 260-267.

Research output: Contribution to journalArticle

Li, Zhongkui ; Khaletskiy, Alexander ; Wang, Jianyi ; Wong, Jeffrey Y C ; Oberley, Larry W. ; Li, Jian-Jian. / Genes regulated in human breast cancer cells overexpressing manganese-containing superoxide dismutase. In: Free Radical Biology and Medicine. 2001 ; Vol. 30, No. 3. pp. 260-267.
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AB - The mitochondrial antioxidant enzyme manganese-containing superoxide dismutase (MnSOD) functions as a tumor suppressor gene. Reconstitution of MnSOD expression in several human cancer cell lines leads to reversion of malignancy and induces a resistant phenotype to the cytotoxic effects of TNF and hyperthermia. The signaling pathways that underlie these phenotypic changes in MnSOD-overexpressing cells are unknown, although alterations in the activity of several redox-sensitive transcription factors, including AP-1 and NF-κB, have been observed. To determine the downstream signaling molecules involved in MnSOD-induced cell resistant phenotype, in the present study we analyzed the expression profile of several groups of genes related to stress response, DNA repair, and apoptosis, in a human breast cancer MCF-7 cell line overexpressing MnSOD (MCF+SOD). Of 588 genes examined, 5 (0.85%) were up-regulated (2-42-fold), and 11 (1.9%) were down-regulated (2-33-fold) in the MCF+SOD cells compared to the parental MCF-7 cells. The five up-regulated genes were MET, GADD153, CD9, α-catenin and plakoglobin. The genes with the most significant down-regulation included: vascular endothelial growth factor receptor 1, TNF-α converting enzyme, and interleukin-1β. GADD153 (involved in the repair of DNA double strand breaks) showed a 33-fold increase in microarray analysis and these results were confirmed by RT-PCR. To further determine the specificity in MnSOD-induced gene regulation, MCF+SOD cells were stably transfected with an antisense MnSOD sequence whose expression was controlled by a tetracycline-inducible regulator. Expression of three up-regulated genes was measured after induction of antisense MnSOD expression. Interestingly, expression level of GADD153 but not MET or CD9 was reduced 24 h after antisense MnSOD induction. Together, these results suggest that reconstitution of MnSOD in tumor cells can specifically modulate the expression of down-stream effector genes. GADD153 and other elements observed in the MCF+SOD cells may play a key role in signaling the MnSOD-induced cell phenotypic change.

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