Generation of oxidant response to copper and iron nanoparticles and salts

Stimulation by ascorbate

Robert H. Rice, Edgar A. Vidrio, Benjamin M. Kumfer, Qin Qin, Neil H. Willits, Ian M. Kennedy, Cort Anastasio

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The present work describes a two-stage approach to analyzing combustion-generated samples for their potential to produce oxidant stress. This approach is illustrated with the two commonly encountered transition metals, copper and iron. First, their abilities to generate hydroxyl radical were measured in a cell-free, phosphate-buffered saline solution containing ascorbate and/or citrate. Second, their abilities to induce heme oxygenase-1 in cultured human epidermal keratinocytes were assessed in cell culture. Combustion-generated copper oxide nanoparticles were active in both assays and were found to be soluble in culture medium. Depletion of glutathione in the cells or loading the cells with ascorbate greatly increased heme oxygenase-1 induction in the presence of copper. By contrast, iron oxide nanoparticles were active in the phosphate-buffered saline but not in cell culture, and they aggregated in culture medium. Soluble salts of copper and iron exhibited the same contrast in activities as the respective combustion-generated particles. The results suggest that the capability of combustion-generated environmental samples to produce oxidant stress can be screened effectively in a two step process, first in phosphate-buffered saline with ascorbate and subsequently in epithelial cell culture for those exhibiting activity initially. The results also point to an unanticipated interaction in cells of oxidant stress-generating metals with an antioxidant (ascorbate) that is usually missing in culture medium formulations. Thus, ascorbate supplementation of cultured human cells is likely to improve their ability to model the in vivo effects of particulate matter containing copper and other redox-active metals.

Original languageEnglish (US)
Pages (from-to)359-365
Number of pages7
JournalChemico-Biological Interactions
Volume181
Issue number3
DOIs
StatePublished - Oct 30 2009

Fingerprint

Oxidants
Nanoparticles
Copper
Iron
Salts
Cell culture
Culture Media
Heme Oxygenase-1
Phosphates
Cell Culture Techniques
Metals
Particulate Matter
Copper oxides
Sodium Chloride
Citric Acid
Hydroxyl Radical
Transition metals
Glutathione
Assays
Keratinocytes

Keywords

  • Heme oxygenase-1
  • Human keratinocytes
  • Hydroxyl radical
  • Nrf2 transcription factor

ASJC Scopus subject areas

  • Toxicology

Cite this

Rice, R. H., Vidrio, E. A., Kumfer, B. M., Qin, Q., Willits, N. H., Kennedy, I. M., & Anastasio, C. (2009). Generation of oxidant response to copper and iron nanoparticles and salts: Stimulation by ascorbate. Chemico-Biological Interactions, 181(3), 359-365. https://doi.org/10.1016/j.cbi.2009.08.007

Generation of oxidant response to copper and iron nanoparticles and salts : Stimulation by ascorbate. / Rice, Robert H.; Vidrio, Edgar A.; Kumfer, Benjamin M.; Qin, Qin; Willits, Neil H.; Kennedy, Ian M.; Anastasio, Cort.

In: Chemico-Biological Interactions, Vol. 181, No. 3, 30.10.2009, p. 359-365.

Research output: Contribution to journalArticle

Rice, RH, Vidrio, EA, Kumfer, BM, Qin, Q, Willits, NH, Kennedy, IM & Anastasio, C 2009, 'Generation of oxidant response to copper and iron nanoparticles and salts: Stimulation by ascorbate', Chemico-Biological Interactions, vol. 181, no. 3, pp. 359-365. https://doi.org/10.1016/j.cbi.2009.08.007
Rice, Robert H. ; Vidrio, Edgar A. ; Kumfer, Benjamin M. ; Qin, Qin ; Willits, Neil H. ; Kennedy, Ian M. ; Anastasio, Cort. / Generation of oxidant response to copper and iron nanoparticles and salts : Stimulation by ascorbate. In: Chemico-Biological Interactions. 2009 ; Vol. 181, No. 3. pp. 359-365.
@article{4aa96f583245469d9dc8998d6651ea44,
title = "Generation of oxidant response to copper and iron nanoparticles and salts: Stimulation by ascorbate",
abstract = "The present work describes a two-stage approach to analyzing combustion-generated samples for their potential to produce oxidant stress. This approach is illustrated with the two commonly encountered transition metals, copper and iron. First, their abilities to generate hydroxyl radical were measured in a cell-free, phosphate-buffered saline solution containing ascorbate and/or citrate. Second, their abilities to induce heme oxygenase-1 in cultured human epidermal keratinocytes were assessed in cell culture. Combustion-generated copper oxide nanoparticles were active in both assays and were found to be soluble in culture medium. Depletion of glutathione in the cells or loading the cells with ascorbate greatly increased heme oxygenase-1 induction in the presence of copper. By contrast, iron oxide nanoparticles were active in the phosphate-buffered saline but not in cell culture, and they aggregated in culture medium. Soluble salts of copper and iron exhibited the same contrast in activities as the respective combustion-generated particles. The results suggest that the capability of combustion-generated environmental samples to produce oxidant stress can be screened effectively in a two step process, first in phosphate-buffered saline with ascorbate and subsequently in epithelial cell culture for those exhibiting activity initially. The results also point to an unanticipated interaction in cells of oxidant stress-generating metals with an antioxidant (ascorbate) that is usually missing in culture medium formulations. Thus, ascorbate supplementation of cultured human cells is likely to improve their ability to model the in vivo effects of particulate matter containing copper and other redox-active metals.",
keywords = "Heme oxygenase-1, Human keratinocytes, Hydroxyl radical, Nrf2 transcription factor",
author = "Rice, {Robert H.} and Vidrio, {Edgar A.} and Kumfer, {Benjamin M.} and Qin Qin and Willits, {Neil H.} and Kennedy, {Ian M.} and Cort Anastasio",
year = "2009",
month = "10",
day = "30",
doi = "10.1016/j.cbi.2009.08.007",
language = "English (US)",
volume = "181",
pages = "359--365",
journal = "Chemico-Biological Interactions",
issn = "0009-2797",
publisher = "Elsevier Ireland Ltd",
number = "3",

}

TY - JOUR

T1 - Generation of oxidant response to copper and iron nanoparticles and salts

T2 - Stimulation by ascorbate

AU - Rice, Robert H.

AU - Vidrio, Edgar A.

AU - Kumfer, Benjamin M.

AU - Qin, Qin

AU - Willits, Neil H.

AU - Kennedy, Ian M.

AU - Anastasio, Cort

PY - 2009/10/30

Y1 - 2009/10/30

N2 - The present work describes a two-stage approach to analyzing combustion-generated samples for their potential to produce oxidant stress. This approach is illustrated with the two commonly encountered transition metals, copper and iron. First, their abilities to generate hydroxyl radical were measured in a cell-free, phosphate-buffered saline solution containing ascorbate and/or citrate. Second, their abilities to induce heme oxygenase-1 in cultured human epidermal keratinocytes were assessed in cell culture. Combustion-generated copper oxide nanoparticles were active in both assays and were found to be soluble in culture medium. Depletion of glutathione in the cells or loading the cells with ascorbate greatly increased heme oxygenase-1 induction in the presence of copper. By contrast, iron oxide nanoparticles were active in the phosphate-buffered saline but not in cell culture, and they aggregated in culture medium. Soluble salts of copper and iron exhibited the same contrast in activities as the respective combustion-generated particles. The results suggest that the capability of combustion-generated environmental samples to produce oxidant stress can be screened effectively in a two step process, first in phosphate-buffered saline with ascorbate and subsequently in epithelial cell culture for those exhibiting activity initially. The results also point to an unanticipated interaction in cells of oxidant stress-generating metals with an antioxidant (ascorbate) that is usually missing in culture medium formulations. Thus, ascorbate supplementation of cultured human cells is likely to improve their ability to model the in vivo effects of particulate matter containing copper and other redox-active metals.

AB - The present work describes a two-stage approach to analyzing combustion-generated samples for their potential to produce oxidant stress. This approach is illustrated with the two commonly encountered transition metals, copper and iron. First, their abilities to generate hydroxyl radical were measured in a cell-free, phosphate-buffered saline solution containing ascorbate and/or citrate. Second, their abilities to induce heme oxygenase-1 in cultured human epidermal keratinocytes were assessed in cell culture. Combustion-generated copper oxide nanoparticles were active in both assays and were found to be soluble in culture medium. Depletion of glutathione in the cells or loading the cells with ascorbate greatly increased heme oxygenase-1 induction in the presence of copper. By contrast, iron oxide nanoparticles were active in the phosphate-buffered saline but not in cell culture, and they aggregated in culture medium. Soluble salts of copper and iron exhibited the same contrast in activities as the respective combustion-generated particles. The results suggest that the capability of combustion-generated environmental samples to produce oxidant stress can be screened effectively in a two step process, first in phosphate-buffered saline with ascorbate and subsequently in epithelial cell culture for those exhibiting activity initially. The results also point to an unanticipated interaction in cells of oxidant stress-generating metals with an antioxidant (ascorbate) that is usually missing in culture medium formulations. Thus, ascorbate supplementation of cultured human cells is likely to improve their ability to model the in vivo effects of particulate matter containing copper and other redox-active metals.

KW - Heme oxygenase-1

KW - Human keratinocytes

KW - Hydroxyl radical

KW - Nrf2 transcription factor

UR - http://www.scopus.com/inward/record.url?scp=70249151058&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70249151058&partnerID=8YFLogxK

U2 - 10.1016/j.cbi.2009.08.007

DO - 10.1016/j.cbi.2009.08.007

M3 - Article

VL - 181

SP - 359

EP - 365

JO - Chemico-Biological Interactions

JF - Chemico-Biological Interactions

SN - 0009-2797

IS - 3

ER -