Generation of interleukin-17 receptor-like protein (IL-17RL) in prostate by alternative splicing of RNA

Dominik R Haudenschild, Shane B. Curtiss, Timothy A. Moseley, A Hari Reddi

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

BACKGROUND. Interleukin-17 receptor-like protein (IL-17RL) expressed in prostate tissues changes with advanced cancers due to extensive alternative splicing, which affects the final protein. Predominant IL-17RL splice isoform variants have not been identified, hindering functional studies. METHODS. A cDNA library of IL-17RL transcripts was arrayed onto nylon membranes. Individual transcript exon structures were determined by successively probing membranes with exon-specific oligonucleotides. The most common variants were transiently over-expressed in 293T cells. RESULTS. We detected >90 different IL-17RL isoforms. Three most abundant isoforms account for approximately half the total transcripts; the full-length variant just over 11%. Surprisingly, most alternative splicing does not alter the reading frame of the full-length molecule; therefore, resulting proteins vary mostly in N-terminal domains. CONCLUSIONS. IL-17RL exists as multiple isoforms due to extensive alternative splicing. We identified the most abundant splices in prostate tissue and established a technique to investigate changes in RNA IL-17RL splicing that occur in advanced cancers.

Original languageEnglish (US)
Pages (from-to)1268-1274
Number of pages7
JournalProstate
Volume66
Issue number12
DOIs
StatePublished - Sep 1 2006

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Keywords

  • Alternative splicing
  • Gleason grade
  • IL-17RC
  • IL-17RL
  • Prostate cancer

ASJC Scopus subject areas

  • Urology

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