Generation of human vascularized brain organoids

Missy T. Pham, Kari M. Pollock, Melanie D. Rose, Whitney A. Cary, Heather R. Stewart, Ping Zhou, Jan Nolta, Ben Waldau

Research output: Contribution to journalArticle

68 Scopus citations

Abstract

The aim of this study was to vascularize brain organoids with a patient's own endothelial cells (ECs). Induced pluripotent stem cells (iPSCs) of one UC Davis patient were grown into whole-brain organoids. Simultaneously, iPSCs from the same patient were differentiated into ECs. On day 34, the organoid was re-embedded in Matrigel with 250 000 ECs. Vascularized organoids were grown in vitro for 3-5 weeks or transplanted into immunodeficient mice on day 54, and animals were perfused on day 68. Coating of brain organoids on day 34 with ECs led to robust vascularization of the organoid after 3-5 weeks in vitro and 2 weeks in vivo. Human CD31-positive blood vessels were found inside and in-between rosettes within the center of the organoid after transplantation. Vascularization of brain organoids with a patient's own iPSC-derived ECs is technically feasible.

Original languageEnglish (US)
Pages (from-to)588-593
Number of pages6
JournalNeuroReport
Volume29
Issue number7
DOIs
StatePublished - Jan 1 2018

Keywords

  • Blood vessel
  • Brain organoid
  • CD31
  • STEM121
  • Transplantation
  • Vascularization

ASJC Scopus subject areas

  • Neuroscience(all)

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    Pham, M. T., Pollock, K. M., Rose, M. D., Cary, W. A., Stewart, H. R., Zhou, P., Nolta, J., & Waldau, B. (2018). Generation of human vascularized brain organoids. NeuroReport, 29(7), 588-593. https://doi.org/10.1097/WNR.0000000000001014