Generation and biological properties of a monoclonal antibody to Galactocerebroside

Abdolmohammad Rostami, P. Ann Eccleston, Donald H. Silberberg, Mikio Hirayama, Robert P. Lisak, David E Pleasure, S. Michael Phillips

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Galactocerebroside (GalC) is a major glycolipid of myelin and myelin-forming cells. We have generated a mouse IgM monoclonal antibody to GalC (M-anti-GalC) which bound only to oligodendrocytes in rat and bovine central nervous system cultures as assessed by immunofluorescence. Double staining with rabbit anti-glial fibrillary acidic protein and anti-fibronectin antisera revealed no binding of M-anti-GalC to astrocytes or fibroblasts. Schwann cells, but not fibroblasts, were stained in short-term cultures of rat Schwann cells. M-anti-GalC exhibited in vitro cytotoxicity to rat and bovine oligodendrocytes in the presence of complement. This monoclonal antibody with its monospecificity, consistent titer, and capacity to induce cell lysis should be useful for in vitro and in vivo investigations concerning myelination and demyelination.

Original languageEnglish (US)
Pages (from-to)203-208
Number of pages6
JournalBrain Research
Volume298
Issue number2
DOIs
StatePublished - Apr 30 1984
Externally publishedYes

Keywords

  • anti-galactocerebroside antibody
  • hybridoma
  • immunofluorescence
  • oligodendrocytes
  • radioimmunoassay
  • Schwann cells

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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    Rostami, A., Eccleston, P. A., Silberberg, D. H., Hirayama, M., Lisak, R. P., Pleasure, D. E., & Phillips, S. M. (1984). Generation and biological properties of a monoclonal antibody to Galactocerebroside. Brain Research, 298(2), 203-208. https://doi.org/10.1016/0006-8993(84)91419-7