Generating somatic mosaicism with a Cre recombinase-microsatellite sequence transgene

Aytekin Akyol, Takao Hinoi, Ying Feng, Guido T. Bommer, Thomas M Glaser, Eric R. Fearon

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


Strategies for altering constitutional or somatic genotype in mice are well established, but approaches to generate mosaic genotypes in mouse tissues are limited. We showed that a functionally inactive Cre recombinase transgene with a long mononucleotide tract altering the reading frame was stochastically activated in the mouse intestinal tract. We demonstrated the utility of this approach by inducing colonic polyposis after Cre-mediated bi-allelic inactivation of the Apc gene.

Original languageEnglish (US)
Pages (from-to)231-233
Number of pages3
JournalNature Methods
Issue number3
StatePublished - Mar 2008
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Cell Biology


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