Generating somatic mosaicism with a Cre recombinase-microsatellite sequence transgene

Aytekin Akyol, Takao Hinoi, Ying Feng, Guido T. Bommer, Thomas M Glaser, Eric R. Fearon

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Strategies for altering constitutional or somatic genotype in mice are well established, but approaches to generate mosaic genotypes in mouse tissues are limited. We showed that a functionally inactive Cre recombinase transgene with a long mononucleotide tract altering the reading frame was stochastically activated in the mouse intestinal tract. We demonstrated the utility of this approach by inducing colonic polyposis after Cre-mediated bi-allelic inactivation of the Apc gene.

Original languageEnglish (US)
Pages (from-to)231-233
Number of pages3
JournalNature Methods
Volume5
Issue number3
DOIs
StatePublished - Mar 2008
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Cell Biology

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