Generating mouse models for biomedical research: technological advances

Channabasavaiah B. Gurumurthy, Kevin C K Lloyd

Research output: Contribution to journalReview article

10 Scopus citations

Abstract

Over the past decade, new methods and procedures have been developed to generate genetically engineered mouse models of human disease. This At a Glance article highlights several recent technical advances in mouse genome manipulation that have transformed our ability to manipulate and study gene expression in the mouse. We discuss how conventional gene targeting by homologous recombination in embryonic stem cells has given way to more refined methods that enable allele-specific manipulation in zygotes. We also highlight advances in the use of programmable endonucleases that have greatly increased the feasibility and ease of editing the mouse genome. Together, these and other technologies provide researchers with the molecular tools to functionally annotate the mouse genome with greater fidelity and specificity, as well as to generate new mouse models using faster, simpler and less costly techniques.

Original languageEnglish (US)
JournalDisease models & mechanisms
Volume12
Issue number1
DOIs
StatePublished - Jan 8 2019

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Keywords

  • CRISPR
  • Genome editing
  • Mouse
  • Mutagenesis

ASJC Scopus subject areas

  • Neuroscience (miscellaneous)
  • Medicine (miscellaneous)
  • Immunology and Microbiology (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)

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