Generating encoded compound libraries for fabricating microarrays as a high-throughput protein ligand discovery platform

Yung Shin Sun, Yiyan Fei, Juntao Luo, Seth Dixon, James P. Landry, Kit Lam, Xiangdong Zhu

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

We demonstrate an effective method for generating libraries of encoded compounds for fabricating large compound microarrays on solid supports. This method is based on one-bead, one-compound synthesis and employs a novel trilayer bead-partition scheme that ensures sufficient quantity of synthesized compounds releasable from each bead for compound microarray fabrication in high-throughput protein-ligand discovery assays. [Supplementary materials are available for this article. Go to the publisher's online edition of Synthetic Communications® for the following free supplemental resource(s): Full experimental and spectral details.]

Original languageEnglish (US)
Pages (from-to)987-1001
Number of pages15
JournalSynthetic Communications
Volume44
Issue number7
DOIs
StatePublished - Apr 3 2014

Keywords

  • Chemical microarray
  • high-throughput screening
  • one-bead-one-compound (OBOC) synthesis
  • protein-ligand binding assay

ASJC Scopus subject areas

  • Organic Chemistry

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