General Tolerance of Galactosyltransferases toward UDP-galactosamine Expands Their Synthetic Capability

Xuan Fu, Madhusudhan Reddy Gadi, Shuaishuai Wang, Jinghua Han, Ding Liu, Xi Chen, Jun Yin, Lei Li

Research output: Contribution to journalArticlepeer-review

Abstract

Accessing large numbers of structurally diverse glycans and derivatives is essential to functional glycomics. We showed a general tolerance of galactosyltransferases toward uridine-diphosphate-galactosamine (UDP-GalN), which is not a commonly used sugar nucleotide donor. The property was harnessed to develop a two-step chemoenzymatic strategy for facile synthesis of novel and divergent N-acetylgalactosamine (GalNAc)-glycosides and derivatives in preparative scales. The discovery and the application of the new property of existing glycosyltransferases expand their catalytic capabilities in generating novel carbohydrate linkages, thus prompting the synthesis of diverse glycans and glycoconjugates for biological studies.

Original languageEnglish (US)
Pages (from-to)26555-26560
Number of pages6
JournalAngewandte Chemie - International Edition
Volume60
Issue number51
DOIs
StatePublished - Dec 13 2021

Keywords

  • galactosyltransferase
  • GalNAc-glycosides
  • general tolerance
  • UDP-galactosamine

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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